Manufacturing protein-DNA chip for depigmenting agent screening = 전사인자 저해제 통한 미백제 탐색용 단백질 칩 제작

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dc.contributor.authorJ S Han-
dc.contributor.authorE Y Kwak-
dc.contributor.authorH B Lee-
dc.contributor.authorJ H Shin-
dc.contributor.authorSeung Hak Baek-
dc.contributor.authorBong Hyun Chung-
dc.contributor.authorE K Kim-
dc.date.accessioned2017-04-19T09:02:21Z-
dc.date.available2017-04-19T09:02:21Z-
dc.date.issued2004-
dc.identifier.issn1226-2587-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6839-
dc.description.abstractAn attempt was made to develop a proteinchip for screening of MITF (microphthalmia transcription factor) inhibitor. Binding of MITF to E-box causes transcription of several pigmenting genes including tyrosinase gene. We investigated binding of MITF and its DNA binding site (E-box) using a protein-DNA chip with various detection methods including flurorescence (Cyt3). SPR (surface plasmon resonance) and SPRi (surface plasmon resonance imaging). A fusion protein (MITF-Maltose Binding Protein) was attached on the glass plate by chemical modification. An inhibitory synthetic DNA oligomer, artificially designed based on the E-box sequence, inhibited the binding of MITF and E-box. These results showed the potentials of flurorescence-based MITF protein chip as a microarray for high throughput screening (HTS) system of depigmenting agents.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleManufacturing protein-DNA chip for depigmenting agent screening = 전사인자 저해제 통한 미백제 탐색용 단백질 칩 제작-
dc.title.alternativeManufacturing protein-DNA chip for depigmenting agent screening-
dc.typeArticle-
dc.citation.titleJournal of Society of Cosmetic Scientists of Korea-
dc.citation.number4-
dc.citation.endPage483-
dc.citation.startPage479-
dc.citation.volume30-
dc.contributor.affiliatedAuthorSeung Hak Baek-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName한정선-
dc.contributor.alternativeName곽은영-
dc.contributor.alternativeName이향복-
dc.contributor.alternativeName신정현-
dc.contributor.alternativeName백승학-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName김은기-
dc.identifier.bibliographicCitationJournal of Society of Cosmetic Scientists of Korea, vol. 30, no. 4, pp. 479-483-
dc.subject.keywordMITF(microphthalmia transcription factor)-
dc.subject.keywordE-box-
dc.subject.keywordβ-CD-
dc.subject.keywordmicroarray-
dc.subject.keywordfluoresence-based detection-
dc.subject.localmicrophthalmia-associated transcription factor (MITF)-
dc.subject.localMITF-
dc.subject.localMitf-
dc.subject.localMITF(microphthalmia transcription factor)-
dc.subject.localE-box-
dc.subject.localβ-CD-
dc.subject.localmicroarray-
dc.subject.localmicroarry-
dc.subject.localMicroarray-
dc.subject.localmicroarrays-
dc.subject.localfluoresence-based detection-
dc.description.journalClassN-
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