DC Field | Value | Language |
---|---|---|
dc.contributor.author | J S Han | - |
dc.contributor.author | E Y Kwak | - |
dc.contributor.author | H B Lee | - |
dc.contributor.author | J H Shin | - |
dc.contributor.author | Seung Hak Baek | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.contributor.author | E K Kim | - |
dc.date.accessioned | 2017-04-19T09:02:21Z | - |
dc.date.available | 2017-04-19T09:02:21Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1226-2587 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6839 | - |
dc.description.abstract | An attempt was made to develop a proteinchip for screening of MITF (microphthalmia transcription factor) inhibitor. Binding of MITF to E-box causes transcription of several pigmenting genes including tyrosinase gene. We investigated binding of MITF and its DNA binding site (E-box) using a protein-DNA chip with various detection methods including flurorescence (Cyt3). SPR (surface plasmon resonance) and SPRi (surface plasmon resonance imaging). A fusion protein (MITF-Maltose Binding Protein) was attached on the glass plate by chemical modification. An inhibitory synthetic DNA oligomer, artificially designed based on the E-box sequence, inhibited the binding of MITF and E-box. These results showed the potentials of flurorescence-based MITF protein chip as a microarray for high throughput screening (HTS) system of depigmenting agents. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Manufacturing protein-DNA chip for depigmenting agent screening = 전사인자 저해제 통한 미백제 탐색용 단백질 칩 제작 | - |
dc.title.alternative | Manufacturing protein-DNA chip for depigmenting agent screening | - |
dc.type | Article | - |
dc.citation.title | Journal of Society of Cosmetic Scientists of Korea | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 483 | - |
dc.citation.startPage | 479 | - |
dc.citation.volume | 30 | - |
dc.contributor.affiliatedAuthor | Seung Hak Baek | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 한정선 | - |
dc.contributor.alternativeName | 곽은영 | - |
dc.contributor.alternativeName | 이향복 | - |
dc.contributor.alternativeName | 신정현 | - |
dc.contributor.alternativeName | 백승학 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.contributor.alternativeName | 김은기 | - |
dc.identifier.bibliographicCitation | Journal of Society of Cosmetic Scientists of Korea, vol. 30, no. 4, pp. 479-483 | - |
dc.subject.keyword | MITF(microphthalmia transcription factor) | - |
dc.subject.keyword | E-box | - |
dc.subject.keyword | β-CD | - |
dc.subject.keyword | microarray | - |
dc.subject.keyword | fluoresence-based detection | - |
dc.subject.local | microphthalmia-associated transcription factor (MITF) | - |
dc.subject.local | MITF | - |
dc.subject.local | Mitf | - |
dc.subject.local | MITF(microphthalmia transcription factor) | - |
dc.subject.local | E-box | - |
dc.subject.local | β-CD | - |
dc.subject.local | microarray | - |
dc.subject.local | microarry | - |
dc.subject.local | Microarray | - |
dc.subject.local | microarrays | - |
dc.subject.local | fluoresence-based detection | - |
dc.description.journalClass | N | - |
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