Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification

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Title
Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification
Author(s)
H S Ro; Sun Ok Jung; Byung Hoon Kho; Hyung Pyo Hong; Jae Sung Lee; Yong Beom Shin; Min-Gon Kim; Bong Hyun Chung
Bibliographic Citation
Applied and Environmental Microbiology, vol. 71, no. 2, pp. 1089-1092
Publication Year
2005
Abstract
A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.
ISSN
0099-2240
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/AEM.71.2.1089-1092.2005
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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