Open Access@KRIBBDivision of Research on National ChallengesBionanotechnology Research Center1. Journal Articles
Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification
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- Title
- Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification
- Author(s)
- H S Ro; Sun Ok Jung; Byung Hoon Kho; Hyung Pyo Hong; Jae Sung Lee; Yong Beom Shin; Min-Gon Kim; Bong Hyun Chung
- Bibliographic Citation
- Applied and Environmental Microbiology, vol. 71, no. 2, pp. 1089-1092
- Publication Year
- 2005
- Abstract
- A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.
- ISSN
- 0099-2240
- Publisher
- Amer Soc Microb
- Full Text Link
- http://dx.doi.org/10.1128/AEM.71.2.1089-1092.2005
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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