High-throughput screening method of inhibitors that block the interaction between 2 helical regions of HIV-1 gp41

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dc.contributor.authorB S Jin-
dc.contributor.authorW K Lee-
dc.contributor.authorK Ahn-
dc.contributor.authorMyung Kyu Lee-
dc.contributor.authorY G Yu-
dc.date.accessioned2017-04-19T09:02:32Z-
dc.date.available2017-04-19T09:02:32Z-
dc.date.issued2005-
dc.identifier.issn1087-0571-
dc.identifier.uri10.1177/1087057104269726ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6859-
dc.description.abstractThe HIV-1 envelope glycoprotein transmembrane subunit, gp41, mediates the fusion of viral and target cell membranes. The 2 helical regions in the ectodomain of gp41, the N-helix and the C-helix, form a helical bundle complex that has been suggested as a fusion-active conformation. Previously, an enzyme-linked immunosorbent assay (ELISA) method had been established to measure the interaction of 2 helical regions of gp41. In this study, the ELISA method was modified to apply high-throughput screening (HTS) of an organic compound library. A few compounds had been identified to prevent the interaction between 2 helical regions of gp41, and they were further shown to inhibit the gp41-mediated viral infection. In addition, they specifically quenched the fluorescence of tryptophan in the N-helix region, indicating that these compounds bound to the N-helix rather than the C-helix of gp41. These results suggested that this assay method targeting gp41 could be used for HTS of HIV fusion inhibitors.-
dc.publisherSage-
dc.titleHigh-throughput screening method of inhibitors that block the interaction between 2 helical regions of HIV-1 gp41-
dc.title.alternativeHigh-throughput screening method of inhibitors that block the interaction between 2 helical regions of HIV-1 gp41-
dc.typeArticle-
dc.citation.titleJournal of Biomolecular Screening-
dc.citation.number1-
dc.citation.endPage19-
dc.citation.startPage13-
dc.citation.volume10-
dc.contributor.affiliatedAuthorMyung Kyu Lee-
dc.contributor.alternativeName진봉석-
dc.contributor.alternativeName이원규-
dc.contributor.alternativeName안광석-
dc.contributor.alternativeName이명규-
dc.contributor.alternativeName유연규-
dc.identifier.bibliographicCitationJournal of Biomolecular Screening, vol. 10, no. 1, pp. 13-19-
dc.identifier.doi10.1177/1087057104269726-
dc.subject.keywordgp41-
dc.subject.keywordHIV-1-
dc.subject.keywordInhibitor-
dc.subject.keywordMembrane fusion-
dc.subject.keywordScreening-
dc.subject.localgp41-
dc.subject.localgp41'-
dc.subject.localHIV-1-
dc.subject.localHIV1-
dc.subject.localinhibitors-
dc.subject.localInhibitors-
dc.subject.localinhibitor-
dc.subject.localInhibitor-
dc.subject.localMembrane fusion-
dc.subject.localScreening-
dc.subject.localscreening-
dc.description.journalClassN-
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