The diversity of culturable organotrophic bacteria from local solar salterns

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Title
The diversity of culturable organotrophic bacteria from local solar salterns
Author(s)
S H Yeon; Won Jin Jeong; J S Park
Bibliographic Citation
Journal of Microbiology, vol. 43, no. 1, pp. 1-10
Publication Year
2005
Abstract
We isolated and cultured bacteria inhabiting solar saltern ponds in Taean-Gun, Chungnam Province, Korea. All of the isolated 64 strains were found to be moderately halophilic bacteria, growing in a salt range of 2-20 %, with an optimal concentration of 5% salt. Bacterial diversity among the isolated halophiles was evaluated via RFLP analyses of PCR-amplified 16S rDNAs, followed by phylogenetic analysis of the partial 16S rDNA sequences. The combination of restriction enzyme digestions with HaeIII, CfoI, MspI and RsaI generated 54 distinct patterns. A neighbor-joining tree of the partial 16S rDNA sequences resulted in the division of the 64 strains into 2 major groups, 45 strains of γ-Proteobacteria (70.3%) and 19 strains of Firmicutes (29.7%). The α-Proteobacteria and Cytophaga-Flavobacterium-Bacterioides groups, which were repeatedly found to exist in thalassohaline environments, were not represented in our isolates. The γ-Proteobacteria group consisted of several subgroups of the Vibrionaceae (37.5%), Pseudoalteromonadaceae (10.9%), Halomonadaceae (7.8%), Alteromonadaceae (7.8%), and Idiomarinaceae (6.3%). Members of Salinivibrio costicola (29.7%) were the most predominant species among all of the isolates, followed by Halobacillus treperi (12.5%). Additionally, three new species candidates were found, based on similarities of the 16S rDNA sequences to those of previously published species.
Keyword
16S rDNAbacterial diversitysolar salternhalophilic bacteria
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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