Genotypic and phenotypic characteristics of Staphylococcus aureus isolates from lettuces and raw milk = 상추와 원유에서 분리한 황색 포도상구균의 유전형 및 표현형 특징

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dc.contributor.authorH J Jung-
dc.contributor.authorJ I Cho-
dc.contributor.authorS H Park-
dc.contributor.authorS D Ha-
dc.contributor.authorK H Lee-
dc.contributor.authorC H Kim-
dc.contributor.authorE S Song-
dc.contributor.authorD H Chung-
dc.contributor.authorMin-Gon Kim-
dc.contributor.authorK Y Kim-
dc.contributor.authorK S Kim-
dc.date.accessioned2017-04-19T09:02:33Z-
dc.date.available2017-04-19T09:02:33Z-
dc.date.issued2005-
dc.identifier.issn0367-6293-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6865-
dc.description.abstractTo characterize genotypic and phenotypic traits of Staphylococcus aureus isolates (n = 86) from lettuces and raw milk, major virulence-associated genes and antibiotic susceptibility were detected using PCR-based methods and disk diffusion method, respectively. All isolates possessed coagulase gene and showed five polymorphism types [500 bp (2.4%), 580 bp (17.4%), 660 bp (61.6%), 740 bp (17.4%), and 820 bp (1.2%)] due to variable numbers of tandem repeats present within the gene. Two or three different loci of hemolysin gene family were dominant in isolates, 47 of which (55%) possessed combination of hla / hld / hlg-2 genes as the most prevalent types. Among enterotoxin-encoding genes, sea was detected from 32 isolates (37%), sed from 1 isolate (1%), and sea and sed genes were co-detected from 4 isolates (5%), whereas seb, sec, and tsst-1 genes were not detected. All isolates were susceptible to ciprofloxacin, trimethoprim/sulfamethoxazole, oxacillin,and vancomycin, 85 isolates (99%) to penicillin G, 54 isolates (63%) to chloramphenicol, 51 isolates (59%) to erythromycin, and 7 isolates (8%) to clindamycin. Among resistant isolates, seven displayed multiantibiotic-resistance against two different antibiotics.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleGenotypic and phenotypic characteristics of Staphylococcus aureus isolates from lettuces and raw milk = 상추와 원유에서 분리한 황색 포도상구균의 유전형 및 표현형 특징-
dc.title.alternativeGenotypic and phenotypic characteristics of Staphylococcus aureus isolates from lettuces and raw milk-
dc.typeArticle-
dc.citation.titleKorean Journal of Food Science & Technology-
dc.citation.number1-
dc.citation.endPage141-
dc.citation.startPage134-
dc.citation.volume37-
dc.contributor.affiliatedAuthorMin-Gon Kim-
dc.contributor.alternativeName정혜진-
dc.contributor.alternativeName조준일-
dc.contributor.alternativeName박성희-
dc.contributor.alternativeName하상도-
dc.contributor.alternativeName이규호-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName송은섭-
dc.contributor.alternativeName정덕화-
dc.contributor.alternativeName김민곤-
dc.contributor.alternativeName김광엽-
dc.contributor.alternativeName김근성-
dc.identifier.bibliographicCitationKorean Journal of Food Science & Technology, vol. 37, no. 1, pp. 134-141-
dc.subject.keywordstaphylococcus aureus-
dc.subject.keywordcoagulase-
dc.subject.keywordhemolysin-
dc.subject.keywordenterotoxin-
dc.subject.keywordantimicrobial susceptibility-
dc.subject.localStaphylococcus aureus-
dc.subject.localstaphylococcus aureus-
dc.subject.localcoagulase-
dc.subject.localhemolysin-
dc.subject.localenterotoxin-
dc.subject.localantimicrobial susceptibility-
dc.description.journalClassN-
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