Identification of recombinant subtilisins

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorNack Shick Choi-
dc.contributor.authorKi Hyun Yoo-
dc.contributor.authorKab Seog Yoon-
dc.contributor.authorKyu Tae Chang-
dc.contributor.authorP J Maeng-
dc.contributor.authorSeung Ho Kim-
dc.date.accessioned2017-04-19T09:02:33Z-
dc.date.available2017-04-19T09:02:33Z-
dc.date.issued2005-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6866-
dc.description.abstractTo identify the activity of recombinant subtilisins (subtilisin BPN' and subtilisin Carlsberg), three different zymography methods, SDS-fibrin zymography (SDS-FZ), reverse fibrin zymography (RFZ), and isoelectric focusing-fibrin zymography (IEF-FZ), were used. The recombinant subtilisins BPN' and Carlsberg did not migrate into the electrophoretic field based on a Laetymili buffer system, instead forming a "binding mode" at the top part of the separating gels with the SDS-FZ and RFZ techniques. Yet, this problem was resolved when using IEF-FZ with a pH range from 3 to 10. In addition, all these methods enabled the activity of a recombinant pro-subtilisin DJ-4 to be detected without a refolding pathway.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleIdentification of recombinant subtilisins-
dc.title.alternativeIdentification of recombinant subtilisins-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number1-
dc.citation.endPage39-
dc.citation.startPage35-
dc.citation.volume15-
dc.contributor.affiliatedAuthorNack Shick Choi-
dc.contributor.affiliatedAuthorKi Hyun Yoo-
dc.contributor.affiliatedAuthorKab Seog Yoon-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName유기현-
dc.contributor.alternativeName윤갑석-
dc.contributor.alternativeName장규태-
dc.contributor.alternativeName맹필재-
dc.contributor.alternativeName김승호-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 15, no. 1, pp. 35-39-
dc.subject.keywordbacillus-
dc.subject.keywordbinding mode-
dc.subject.keywordfibrin zymography-
dc.subject.keywordisoelectric focusing-
dc.subject.keywordrecombinant subtilisin-
dc.subject.localBacillus-
dc.subject.localbacillus-
dc.subject.localbinding mode-
dc.subject.localFibrin zymography-
dc.subject.localfibrin zymography-
dc.subject.localisoelectric focusing-
dc.subject.localrecombinant subtilisin-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.