DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nack Shick Choi | - |
dc.contributor.author | Ki Hyun Yoo | - |
dc.contributor.author | Kab Seog Yoon | - |
dc.contributor.author | Kyu Tae Chang | - |
dc.contributor.author | P J Maeng | - |
dc.contributor.author | Seung Ho Kim | - |
dc.date.accessioned | 2017-04-19T09:02:33Z | - |
dc.date.available | 2017-04-19T09:02:33Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6866 | - |
dc.description.abstract | To identify the activity of recombinant subtilisins (subtilisin BPN' and subtilisin Carlsberg), three different zymography methods, SDS-fibrin zymography (SDS-FZ), reverse fibrin zymography (RFZ), and isoelectric focusing-fibrin zymography (IEF-FZ), were used. The recombinant subtilisins BPN' and Carlsberg did not migrate into the electrophoretic field based on a Laetymili buffer system, instead forming a "binding mode" at the top part of the separating gels with the SDS-FZ and RFZ techniques. Yet, this problem was resolved when using IEF-FZ with a pH range from 3 to 10. In addition, all these methods enabled the activity of a recombinant pro-subtilisin DJ-4 to be detected without a refolding pathway. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Identification of recombinant subtilisins | - |
dc.title.alternative | Identification of recombinant subtilisins | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 39 | - |
dc.citation.startPage | 35 | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Nack Shick Choi | - |
dc.contributor.affiliatedAuthor | Ki Hyun Yoo | - |
dc.contributor.affiliatedAuthor | Kab Seog Yoon | - |
dc.contributor.affiliatedAuthor | Kyu Tae Chang | - |
dc.contributor.affiliatedAuthor | Seung Ho Kim | - |
dc.contributor.alternativeName | 최낙식 | - |
dc.contributor.alternativeName | 유기현 | - |
dc.contributor.alternativeName | 윤갑석 | - |
dc.contributor.alternativeName | 장규태 | - |
dc.contributor.alternativeName | 맹필재 | - |
dc.contributor.alternativeName | 김승호 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 15, no. 1, pp. 35-39 | - |
dc.subject.keyword | bacillus | - |
dc.subject.keyword | binding mode | - |
dc.subject.keyword | fibrin zymography | - |
dc.subject.keyword | isoelectric focusing | - |
dc.subject.keyword | recombinant subtilisin | - |
dc.subject.local | Bacillus | - |
dc.subject.local | bacillus | - |
dc.subject.local | binding mode | - |
dc.subject.local | Fibrin zymography | - |
dc.subject.local | fibrin zymography | - |
dc.subject.local | isoelectric focusing | - |
dc.subject.local | recombinant subtilisin | - |
dc.description.journalClass | Y | - |
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