Production of active carboxypeptidase Y of Saccharomyces cerevisiae secreted from methylotrophic yeast Pichia pastoris

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dc.contributor.authorH S Ro-
dc.contributor.authorMi Sun Lee-
dc.contributor.authorMoon Sun Hahm-
dc.contributor.authorHee Sung Bae-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:02:33Z-
dc.date.available2017-04-19T09:02:33Z-
dc.date.issued2005-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6869-
dc.description.abstractOur previous study showed that the overexpression of carboxypeptidase Y (CPY) of Saccharomyces cerevisiae in Escherichia coli resulted in the formation of insoluble inclusion bodies. To produce soluble CPY, we designed a novel Pichia pastoris expression system in which the following were inserted into expression vectors: three different signal sequences derived from the mating factor a1 of S. cerevisiae, an inulinase of Kluyveromyces marxianus, and the endogenous signal sequence of CPY. The expression vector pHIL-D2-SSinul-proCPY was the most effective in the production of proCPY among the vectors examined. The purified active CPY was obtained from proCPY by treating with proteinase K, followed by Q-Excellose ion-exchange column chromatography.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleProduction of active carboxypeptidase Y of Saccharomyces cerevisiae secreted from methylotrophic yeast Pichia pastoris-
dc.title.alternativeProduction of active carboxypeptidase Y of Saccharomyces cerevisiae secreted from methylotrophic yeast Pichia pastoris-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number1-
dc.citation.endPage205-
dc.citation.startPage202-
dc.citation.volume15-
dc.contributor.affiliatedAuthorMi Sun Lee-
dc.contributor.affiliatedAuthorMoon Sun Hahm-
dc.contributor.affiliatedAuthorHee Sung Bae-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName노현수-
dc.contributor.alternativeName이미선-
dc.contributor.alternativeName함문선-
dc.contributor.alternativeName배희성-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 15, no. 1, pp. 202-205-
dc.subject.keywordcarboxypeptidase-
dc.subject.keywordpichia expression-
dc.subject.keywordproteinase K-
dc.subject.keywordsecretory-
dc.subject.localcarboxypeptidase-
dc.subject.localpichia expression-
dc.subject.localproteinase K-
dc.subject.localsecretory-
dc.description.journalClassY-
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