Analyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032

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dc.contributor.authorMin Woo Moon-
dc.contributor.authorHyo Jin Kim-
dc.contributor.authorTae Kwang Oh-
dc.contributor.authorC S Shin-
dc.contributor.authorJ S Lee-
dc.contributor.authorS J Kim-
dc.contributor.authorJung-Kee Lee-
dc.date.accessioned2017-04-19T09:02:34Z-
dc.date.available2017-04-19T09:02:34Z-
dc.date.issued2005-
dc.identifier.issn0378-1097-
dc.identifier.uri10.1016/j.femsle.2005.01.053ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6873-
dc.description.abstractCorynebacterium glutamicum ATCC 13032 has four enzyme II (EII) genes of the phosphotransferase system in its genome encoding transporters for sucrose, glucose, fructose, and an unidentified EII. To analyze the function of these EII genes, they were inactivated via homologous recombination and the resulting mutants characterized for sugar utilization. Whereas the sucrose EII was the only transport system for sucrose in C. glutamicum, fructose and glucose were each transported by a second transporter in addition to their corresponding EII. In addition, the ptsF ptsG double mutant carrying deletions in the EII genes for fructose and glucose accumulated fructose in the culture broth when growing on sucrose. As no fructokinase gene exists in the C. glutamicum genome, the fructokinase gene from Clostridium acetobutylicum was expressed in C. glutamicum and resulted in the direct phosphorylation of fructose without any fructose efflux. Accordingly, since fructokinase could direct fructose flux to the pentose phosphate pathway for the supply of NADPH, fructokinase expression may be a potential strategy for enhancing amino acid production.-
dc.publisherOxford Univ Press-
dc.titleAnalyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032-
dc.title.alternativeAnalyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032-
dc.typeArticle-
dc.citation.titleFEMS Microbiology Letters-
dc.citation.number2-
dc.citation.endPage266-
dc.citation.startPage259-
dc.citation.volume244-
dc.contributor.affiliatedAuthorMin Woo Moon-
dc.contributor.affiliatedAuthorHyo Jin Kim-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.affiliatedAuthorJung-Kee Lee-
dc.contributor.alternativeName문민우-
dc.contributor.alternativeName김효진-
dc.contributor.alternativeName오태광-
dc.contributor.alternativeName신철수-
dc.contributor.alternativeName이종수-
dc.contributor.alternativeName김성준-
dc.contributor.alternativeName이정기-
dc.identifier.bibliographicCitationFEMS Microbiology Letters, vol. 244, no. 2, pp. 259-266-
dc.identifier.doi10.1016/j.femsle.2005.01.053-
dc.subject.keywordCorynebacterium glutamicum-
dc.subject.keywordEnzyme II-
dc.subject.keywordFructokinase-
dc.subject.keywordPhosphotransferase system-
dc.subject.keywordSugar transport-
dc.subject.localcorynebacterium glutamicum-
dc.subject.localCorynebacterium glutamicum-
dc.subject.localenzyme II-
dc.subject.localEnzyme II-
dc.subject.localFructokinase-
dc.subject.localPhosphotransferase system-
dc.subject.localphosphotransferase system-
dc.subject.localsugar transport-
dc.subject.localSugar transport-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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