Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray

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dc.contributor.authorMin-Gon Kim-
dc.contributor.authorYong Beom Shin-
dc.contributor.authorJin Mi Jung-
dc.contributor.authorH S Ro-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:02:37Z-
dc.date.available2017-04-19T09:02:37Z-
dc.date.issued2005-
dc.identifier.issn00221759-
dc.identifier.uri10.1016/j.jim.2004.12.007ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6890-
dc.description.abstractWe describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-γ at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format.-
dc.publisherElsevier-
dc.titleEnhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray-
dc.title.alternativeEnhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray-
dc.typeArticle-
dc.citation.titleJournal of Immunological Methods-
dc.citation.number1-
dc.citation.endPage132-
dc.citation.startPage125-
dc.citation.volume297-
dc.contributor.affiliatedAuthorYong Beom Shin-
dc.contributor.alternativeName김민곤-
dc.contributor.alternativeName신용범-
dc.contributor.alternativeName정진미-
dc.contributor.alternativeName노현수-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationJournal of Immunological Methods, vol. 297, no. 1, pp. 125-132-
dc.identifier.doi10.1016/j.jim.2004.12.007-
dc.subject.keywordHorseradish peroxidase-
dc.subject.keywordImmunoassay-
dc.subject.keywordInterferon-γ-
dc.subject.keywordInterleukin-5-
dc.subject.keywordSurface plasmon resonance (SPR)-
dc.subject.localHorseradish peroxidase-
dc.subject.localImmunoassay-
dc.subject.localInterferon-γ-
dc.subject.localInterleukin-5-
dc.subject.localSurface plasmon resonance (SPR)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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