Open Access@KRIBBDivision of Research on National ChallengesBionanotechnology Research Center1. Journal Articles
Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Min-Gon Kim | - |
| dc.contributor.author | Yong Beom Shin | - |
| dc.contributor.author | Jin Mi Jung | - |
| dc.contributor.author | H S Ro | - |
| dc.contributor.author | Bong Hyun Chung | - |
| dc.date.accessioned | 2017-04-19T09:02:37Z | - |
| dc.date.available | 2017-04-19T09:02:37Z | - |
| dc.date.issued | 2005 | - |
| dc.identifier.issn | 0022-1759 | - |
| dc.identifier.uri | 10.1016/j.jim.2004.12.007 | ko |
| dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6890 | - |
| dc.description.abstract | We describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-γ at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format. | - |
| dc.publisher | Elsevier | - |
| dc.title | Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray | - |
| dc.title.alternative | Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray | - |
| dc.type | Article | - |
| dc.citation.title | Journal of Immunological Methods | - |
| dc.citation.number | 1 | - |
| dc.citation.endPage | 132 | - |
| dc.citation.startPage | 125 | - |
| dc.citation.volume | 297 | - |
| dc.contributor.affiliatedAuthor | Min-Gon Kim | - |
| dc.contributor.affiliatedAuthor | Yong Beom Shin | - |
| dc.contributor.affiliatedAuthor | Jin Mi Jung | - |
| dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
| dc.contributor.alternativeName | 김민곤 | - |
| dc.contributor.alternativeName | 신용범 | - |
| dc.contributor.alternativeName | 정진미 | - |
| dc.contributor.alternativeName | 노현수 | - |
| dc.contributor.alternativeName | 정봉현 | - |
| dc.identifier.bibliographicCitation | Journal of Immunological Methods, vol. 297, no. 1, pp. 125-132 | - |
| dc.identifier.doi | 10.1016/j.jim.2004.12.007 | - |
| dc.subject.keyword | Horseradish peroxidase | - |
| dc.subject.keyword | Immunoassay | - |
| dc.subject.keyword | Interferon-γ | - |
| dc.subject.keyword | Interleukin-5 | - |
| dc.subject.keyword | Surface plasmon resonance (SPR) | - |
| dc.subject.local | horseradish peroxidase | - |
| dc.subject.local | Horseradish peroxidase | - |
| dc.subject.local | Immunoassay | - |
| dc.subject.local | Immunoassays | - |
| dc.subject.local | immunoassay | - |
| dc.subject.local | Interferon γ | - |
| dc.subject.local | interferon-γ | - |
| dc.subject.local | Interferon-γ | - |
| dc.subject.local | interferon-gamma | - |
| dc.subject.local | Interferon gamma | - |
| dc.subject.local | Interleukin-5 | - |
| dc.subject.local | Surface Plasmon Resonance | - |
| dc.subject.local | Surfaceplasmonresonance | - |
| dc.subject.local | Surface plasmon resonance (SPR) | - |
| dc.subject.local | surface plasmon resonance | - |
| dc.subject.local | Surface plasmon resonance | - |
| dc.subject.local | surface plasmon resonance (SPR) | - |
| dc.description.journalClass | Y | - |
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