Gene expression analysis of cultured amniotic fluid cell with down syndrome by DNA microarray

Cited 26 time in scopus
Metadata Downloads
Gene expression analysis of cultured amniotic fluid cell with down syndrome by DNA microarray
I H Chung; S H Lee; K W Lee; S H Park; K Y Cha; Nam-Soon Kim; Hyang Sook Yoo; Yong Sung Kim; S Lee
Bibliographic Citation
Journal of Korean Medical Science, vol. 20, no. 1, pp. 82-87
Publication Year
Complete or partial triplication of human chromosome 21 results in Down syndrome (DS). To analyze differential gene expressions in amniotic fluid (AF) cells of DS, we used a DNA microarray system to analyze 102 genes, which included 24 genes on chromosome 21, 28 genes related to the function of brain and muscle, 36 genes related to apoptosis, 4 genes related to extracellular matrix, 8 genes related to other molecular function and 2 house-keeping genes. AF cells were collected from 12 pregnancies at 16-18 weeks of gestation in DS (n=6) and normal (n=6) subjects. Our DNA microarray experiments showed that the expressions of 11 genes were altered by at least 2-folds in DS, as follows. Ten genes, COL6A1, CASP5, AKT2, JUN, PYGM, BNIP1, OSF-2, PRSS7, COL3A1, and MBLL were down-regulated and GSTT1 was only up-regulated. The differential expressions of GSTT1 and COL3A1 were further confirmed by semi-quantitative RT-PCR for each sample. The gene dosage hypothesis on chromosome 21 may explain the neurological and other symptoms of DS. However, our results showed that only two genes (COL6A1 and PRSS7), among 24 genes on chromosome 21, were down-regulated in the AF cells of DS. Our data may provide the basis for a more systematic identification of biological markers of fetal DS, thus leading to an improved understanding of pathogenesis for fetal DS.
Amniotic FluidDown SyndromeGene ExpressionOligonucleotide Array Sequence Analysis
Korea Soc-Assoc-Inst
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.