EST analysis of gene expression in the germ-layer formation stage of the Bombyx mori

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Title
EST analysis of gene expression in the germ-layer formation stage of the Bombyx mori
Author(s)
S M Hong; S K Nho; Nam-Soon Kim; Jung Hwa Oh; J S Lee; S W Kang
Bibliographic Citation
Genes & Genomics, vol. 27, no. 1, pp. 47-58
Publication Year
2005
Abstract
In order to explore the gene expression underlying early embyogenesis, expressed sequence tag (EST) analysis was conducted using a cDNA library made from the 24h embryo of a silkworm, Bombyx mori. A total of 8,928 clones were screened plasmid with cDNA insert and have partially sequenced the 5′ ends to generate 2,446 assembled sequences. The annotation of 2,446 high-quality ESTs by a BLAST search, revealed that 1,516 (62%) of the sequences represented known genes. Over 2,400 ESTs consisted of 1,568 single ESTs (singlets) and 878 redundant ESTs (contigs). The functional groups of those sequences with matches in the database (P ?
Keyword
bombyx moricell-communicationdevelopmentembryogenesisESTs
ISSN
1976-9571
Publisher
Springer
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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