Production of soluble human interleukin-6 in cytoplasm by fed-batch culture of recombinant E. coli

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dc.contributor.authorTae Wan Kim-
dc.contributor.authorBong Hyun Chung-
dc.contributor.authorY K Chang-
dc.date.accessioned2017-04-19T09:02:43Z-
dc.date.available2017-04-19T09:02:43Z-
dc.date.issued2005-
dc.identifier.issn8756-7938-
dc.identifier.uri10.1021/bp049645jko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6922-
dc.description.abstractThe major objective of this study is to identify fed-batch culture conditions optimal for the production of human interleukin-6 (hIL-6) in a soluble form. Five different expression vectors were constructed for the expression of hIL-6 and hIL-6s fused with NusA, maltose binding protein (MBP), thioredoxin (Trx) or ubiquitin (Ubi). A series of flask cultures were conducted in LB medium at 37°C. The intact hIL-6 was expressed mostly in the form of inclusion body. More than 95% of the hIL-6 fused with NusA (NusA/hIL-6) and about 90% of MBP/hIL-6 were expressed in a soluble form, whereas Trx/hIL-6 and Ubi/hIL-6 were expressed mostly in the form of inclusion body. Based on this result, NusA was selected as the fusion partner for the production of hIL-6 in the subsequent experiments. A series of pH-stat fed-batch cultures of an E. coli BL21(DE3) transformed with a NusA/hIL-6 expression vector were conducted in a bioreactor with a working volume of about 3 L. As the amount of nitrogen source was increased in the feeding medium, more soluble NusA/hIL-6 was produced, while the total amount was not significantly changed. Under the best conditions 1 ested, about 90% of NusA/hIL-6 was produced in the soluble form. In this case, the concentration of soluble NusA/hIL-6 was 7.5 g/L with a volumetric productivity of 0.43 g/L-h.-
dc.publisherWiley-
dc.titleProduction of soluble human interleukin-6 in cytoplasm by fed-batch culture of recombinant E. coli-
dc.title.alternativeProduction of soluble human interleukin-6 in cytoplasm by fed-batch culture of recombinant E. coli-
dc.typeArticle-
dc.citation.titleBiotechnology Progress-
dc.citation.number2-
dc.citation.endPage531-
dc.citation.startPage524-
dc.citation.volume21-
dc.contributor.affiliatedAuthorTae Wan Kim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName김태완-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName장용근-
dc.identifier.bibliographicCitationBiotechnology Progress, vol. 21, no. 2, pp. 524-531-
dc.identifier.doi10.1021/bp049645j-
dc.description.journalClassY-
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