Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase

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Title
Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase
Author(s)
F Z Wang; Q B Wang; Suk Yoon KwonSang Soo Kwak; W A Su
Bibliographic Citation
Journal of Plant Physiology, vol. 162, no. 4, pp. 465-472
Publication Year
2005
Abstract
We investigated the role that manganese superoxide dismutase (MnSOD), an important antioxidant enzyme, may play in the drought tolerance of rice. MnSOD from pea (Pisum sativum) under the control of an oxidative stress-inducible SWPA2 promoter was introduced into chloroplasts of rice (Oryza sativa) by Agrobacterium-mediated transformation to develop drought-tolerant rice plants. Functional expression of the pea MnSOD in transgenic rice plants (T1) was revealed under drought stress induced by polyethylene glycol (PEG) 6000. After PEG treatment the transgenic leaf slices showed reduced electrolyte leakage compared to wild type (WT) leaf slices, whether they were exposed to methyl viologen (MV) or not, suggesting that transgenic plants were more resistant to MV- or PEG-induced oxidative stress. Transgenic plants also exhibited less injury, measured by net photosynthetic rate, when treated with PEG. Our data suggest that SOD is a critical component of the ROS scavenging system in plant chloroplasts and that the expression of MnSOD can improve drought tolerance in rice.
Keyword
DroughtOryza sativaOxidative stressSuperoxide dismutaseTransgene
ISSN
0176-1617
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jplph.2004.09.009
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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