Plant regeneration through somatic embryogenesis of Leymus chinensis Trin. = 양초(Leymus chinensis Trin.)의 체세포배발생에 의한 식물체 재분화

Abstract

Chinese leymus (Leymus chinensis Trin.) is a perennial grass that is widely distributed at high pH sodic and arid soil in the northeastern Asia. An efficient regeneration system was established through somatic embryogenesis of mature seeds to understand its high adaptability to harsh environmental conditions on the basis of molecular biology. The calli were efficiently induced (about ) from mature seeds on MS medium supplemented with 2,4-D. Somatic embryos were formed from the surface of embryogenic callus on MS medium supplemented with NAA after 3 weeks of culture. Roots were induced from the shoot when transferred to MS medium without plant growth regulator for 1 week. Plant regeneration rate was and regenerated plantlets were grown to normal mature plants in pot. An efficient plant regeneration system in this study will be useful for molecular breeding of L. chinensis.