Molecular taxonomy of a soil actinomycete isolate, KCCM10454 showing neuroprotective activity by 16S rRNA and rpoB gene analysis

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Title
Molecular taxonomy of a soil actinomycete isolate, KCCM10454 showing neuroprotective activity by 16S rRNA and rpoB gene analysis
Author(s)
B H Lee; H Kim; H J Kim; Y K Lim; K H Byun; B Hutchinson; Chang-Jin Kim; Y H Ko; K H Lee; C Y Cha; Y H Kook; B J Kim
Bibliographic Citation
Journal of Microbiology, vol. 43, no. 2, pp. 213-218
Publication Year
2005
Abstract
Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4,5-dimethylthiazol-2- yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampin-resistant genotype, Asn(AAC)442, according to the results of 16S rRNA and rpoB gene analyses.
Keyword
antiepileptic activityapoptosisepilepsyhippocampal cellrifampin resistant genotypestreptomyces
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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