Antitumor activity of spinasterol isolated from Pueraria roots = Pueraria 뿌리에서 동정한 spinasterol의 항암효과

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Title
Antitumor activity of spinasterol isolated from Pueraria roots = Pueraria 뿌리에서 동정한 spinasterol의 항암효과
Author(s)
Gook Che Jeon; M S Park; Do Young Yoon; C H Shin; H S Sin; S J Um
Bibliographic Citation
Experimental and Molecular Medicine, vol. 37, no. 2, pp. 111-120
Publication Year
2005
Abstract
We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria Iobata from Korea), which is used as a rejuvenating folk medicine in Thailand and China. Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography. Using the fractions obtained during separation, we first investigated their cytotoxicity in several cancer cell lines from various tissues. The ethanol-extracted components (PE1, PE4) had significant antiproliferative effects on breast cancer cell lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared these results with the cytotoxic effects of known flavonoids, sterols, and coumarins from Pueraria root. The known compounds were not as effective, and occurred in a different polarity region on HPLC. Third, further separation resulted in the isolation of eight different components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cell lines (MCF-7, MDA-MB-231) in a dose- and time-dependent manner, as well as the growth of ovarian (2774) and cervical cancer cells (HeLa). Finally, a transfection assay showed that this component had an estrogenic effect similar to 17β-estradiol, which activates both estrogen receptor α (ERα) and ERβ. The NMR analysis determined that spinasterol (stigmasta-7, 22-dien-3beta-ol) is an active cytotoxic component of Pueraria root.
Keyword
Breast neoplasmsEstrogen replacementPhytoestrogensPuerariaReceptors, estrogenSpinasterolTherapy
ISSN
I000-0028
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/emm.2005.15
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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