The first two-dimensional reference map of the fission yeast, Schizosaccharomyces pombe proteins

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Title
The first two-dimensional reference map of the fission yeast, Schizosaccharomyces pombe proteins
Author(s)
N Sun; J Jang; S Lee; S Kim; S Lee; Kwang Lae Hoe; Kyung Sook ChungDong Uk Kim; Hyang Sook Yoo; Mi Sun Won; K B Song
Bibliographic Citation
Proteomics, vol. 5, no. 6, pp. 1574-1579
Publication Year
2005
Abstract
Cytosolic proteins of Schizosaccharomyces pombe were separated by two-dimensional (2-D) gel electrophoresis, to construct the first 2-D reference map. In the pI range 4-7, more than 500 spots were detected by silver staining, and 70 different proteins corresponding to 111 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and tandem mass spectrometry, where necessary. In the pI range 6-9, approximately 330 spots were detected, and 31 proteins corresponding to 38 spots were identified by mass spectrometry. More than 50% of the identified proteins were involved in amino acid, carbohydrate or nucleotide metabolism, and energy production. A second large group of identified proteins comprises heat shock and other stress related proteins and chaperones.
Keyword
matrix-assisted laser desorption/ionizationpeptide fingerprintingproteomeschizosaccharomyces pombetwo-dimensional gel electrophoresis
ISSN
1615-9853
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/pmic.200401053
Type
Article
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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