A plant EPF-type zinc-finger protein, CaPIF1, involved in defence against pathogens

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Title
A plant EPF-type zinc-finger protein, CaPIF1, involved in defence against pathogens
Author(s)
Sang-Keun Oh; Jeong Mee Park; Young Hee Joung; Sanghyeob Lee; Eunsook Chung; Soo Yong Kim; S H Yu; Doil Choi
Bibliographic Citation
Molecular Plant Pathology, vol. 6, no. 3, pp. 269-285
Publication Year
2005
Abstract
To understand better the defence responses of plants to pathogen attack, we challenged hot pepper plants with bacterial pathogens and identified transcription factor-encoding genes whose expression patterns were altered during the subsequent hypersensitive response. One of these genes, CaPIF1 (Capsicum annuum Pathogen-Induced Factor 1), was characterized further. This gene encodes a plant-specific EPF-type protein that contains two Cys 2/ His2 zinc fingers. CaPIF1 expression was rapidly and specifically induced when pepper plants were challenged with bacterial pathogens to which they are resistant. In contrast, challenge with a pathogen to which the plants are susceptible only generated weak CaPIF1 expression. CaPIF1 expression was also strongly induced in pepper leaves by the exogenous application of ethephon, an ethylene-releasing compound, and salicylic acid, whereas methyl jasmonate had only moderate effects. CaPIF1 localized to the nuclei of onion epidermis when expressed as a CaPIF1-smGFP fusion protein. Transgenic tobacco plants over-expressing CaPIF1 driven by the CaMV 35S promoter showed increased resistance to challenge with a tobacco-specific pathogen or non-host bacterial pathogens. These plants also showed constitutive up-regulation of multiple defence-related genes. Moreover, virus-induced silencing of the CaPIF1 orthologue in Nicotiana benthamiana enhanced susceptibility to the same host or non-host bacterial pathogens. These observations provide evidence that an EPF-type Cys2/His2 zinc-finger protein plays a crucial role in the activation of the pathogen defence response in plants.
ISSN
1464-6722
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1364-3703.2005.00284.x
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > International Biological Material Research Center > 1. Journal Articles
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