Tumor suppressor VDUP1 increases p27kip1 stability by inhibiting JAB1

Abstract

Vitamin D3 up-regulated protein 1 (VDUP1) is a stress-response gene that is up-regulated by 1,25(OH)3D3 in many cells. It has been reported that VDUP1 expression is reduced in many tumor cells and the enforced expression of VDUP1 inhibits cell proliferation by arresting cell cycle progression. Here, we found that VDUP1-/- fibroblast cells proliferated more rapidly compared with wild-type cells with reduced expression of p27kip1, a cyclin-dependent kinase inhibitor. JAB1 is known to interact with p27kip1 and to decrease the stability of p27 kip1. VDUP1 interacted with JAB1 and restored JAB1-induced suppression of p27kip1 stability. In this process, VDUP1 blocked the JAB1-mediated translocation of p27kip1 from the nucleus to the cytoplasm. In addition, VDUP1 inhibited JAB1-mediated activator protein-1 activation and cell proliferation. Taken together, these results indicate that VDUP1 is a novel factor of p27kip1 stability via regulating JAB1.