A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines

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A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines
H B Lee; Y Kim; H Z Jin; Jung Joon Lee; Chang-Jin Kim; J Y Park; H S Jung
Bibliographic Citation
Letters in Applied Microbiology, vol. 40, no. 6, pp. 497-503
Publication Year
Aims: To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines. Methods and Results: A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI- or HRFAB-MS and 1H and 13C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC50 value of 0.65 and 5.07 μg ml-1 respectively. Conclusions: Harzianum A with a chemical formula of C23H 28O6 was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human cancer cell lines. Significance and Impact of the Study: This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.
Tumour cell lineCytotoxicityHarzianum AHypocreaMycotoxinTrichoderma
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