Genetic and sequence analysis of the RAPD fingerprint containing a Rsv3-linked marker rapidly identified by using RILs in soybean

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dc.contributor.authorSeok Yu-
dc.contributor.authorJ K Moon-
dc.contributor.authorT Y Hwang-
dc.contributor.authorY H Kim-
dc.contributor.authorHwan Mook Kim-
dc.contributor.authorSoon Chun Jeong-
dc.date.accessioned2017-04-19T09:03:00Z-
dc.date.available2017-04-19T09:03:00Z-
dc.date.issued2005-
dc.identifier.issn19769571-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7002-
dc.description.abstractSoybean mosaic virus (SMV) is a prevalent virus that infects soybean [Glycine max (L.) Merr.] crops worldwide. The Rsv3 gene of soybean confers resistance to three (G5-G7) of the most virulent strains of 7 defined SMV strains, which were grouped based on virus-reaction phenotypes of a spectrum of soybean cultivars mainly carrying Rsv1 alleles. The objective of this study was to develop high throughput molecular markers tightly linked to Rsv3 for marker-assisted selection by the random amplified polymorphic DNA (RAPD) fingerprinting method. A recombinant inbred line (RIL) population from a cross between 'IT182932' and 'Hwangkeumkong' and a BC3F2 population from a cross between 'L29' (resistant) and 'Sowonkong' (susceptible) were primarily used. Two hundred twenty oligonucleotide primers (10mer) were run in a set of recombinant individuals. A polymorphic DNA fragment generated by OP-Z20 was tightly linked to Rsv3 in the analysis of the BC3F 2 population. The fragment was subsequently sequenced and converted to a semi-codominant marker. Sequence analysis of a common band generated by OP-Z20 revealed similarity to the sequence of transposase, characteristic of the Mutator transposable element system. The results could facilitate marker-assisted selection to pyramid Rsv3 with other SMV resistance genes into elite soybean cultivars.-
dc.publisherSpringer-
dc.titleGenetic and sequence analysis of the RAPD fingerprint containing a Rsv3-linked marker rapidly identified by using RILs in soybean-
dc.title.alternativeGenetic and sequence analysis of the RAPD fingerprint containing a Rsv3-linked marker rapidly identified by using RILs in soybean-
dc.typeArticle-
dc.citation.titleGenes & Genomics-
dc.citation.number2-
dc.citation.endPage132-
dc.citation.startPage127-
dc.citation.volume27-
dc.contributor.affiliatedAuthorSoon Chun Jeong-
dc.contributor.alternativeName유석-
dc.contributor.alternativeName문정경-
dc.contributor.alternativeName황태영-
dc.contributor.alternativeName김열호-
dc.contributor.alternativeName김환묵-
dc.contributor.alternativeName정순천-
dc.identifier.bibliographicCitationGenes & Genomics, vol. 27, no. 2, pp. 127-132-
dc.subject.keywordmarker-assisted selection-
dc.subject.keywordRAPD-
dc.subject.keywordSMV-
dc.subject.keywordsoybean-
dc.subject.keywordtransposable element-
dc.subject.localmarker-assisted selection-
dc.subject.localMarker-assisted selection-
dc.subject.localRAPD-
dc.subject.localSMV-
dc.subject.localsoybean-
dc.subject.localSoybean-
dc.subject.localtransposable element-
dc.subject.localTransposable element-
dc.description.journalClassY-
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Ochang Branch Institute > 1. Journal Articles
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