TNF-α activates death pathway in human aorta smooth muscle cell in the presence of 7-ketocholesterol

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dc.contributor.authorHyun Sun Lee-
dc.contributor.authorJong Sun Chang-
dc.contributor.authorJin Ah Baek-
dc.contributor.authorMi Yeon Chung-
dc.contributor.authorH C Lee-
dc.contributor.authorB Y Rhim-
dc.contributor.authorD E Sok-
dc.contributor.authorMun Chual Rho-
dc.contributor.authorYoung Kook Kim-
dc.contributor.authorK Kim-
dc.date.accessioned2017-04-19T09:03:00Z-
dc.date.available2017-04-19T09:03:00Z-
dc.date.issued2005-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2005.05.196ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7003-
dc.description.abstractThis study was undertaken to investigate whether a physiologically compatible concentration of 7-ketocholesterol had any effect on human vascular smooth muscle cells (HVSMCs). We found that 7-ketocholesterol changed the viability of human aorta smooth muscle cells (HAoSMC) not by cytotoxicity but by activation of tumor necrosis factor-α receptor (TNFR)-mediated death. Whereas TNF-α did not affect the viability in the presence of 7α-hydroxycholesterol or cholesterol, the cytokine induced HAoSMC death in the presence of 7-ketocholesterol as detected by morphology, viability, and fragmentation of chromosomal DNA. The HAoSMC death was inhibited by a neutralizing anti-TNF receptor 1 (TNFR1) antibody and by the caspase inhibitors of z-VAD and z-DEVD. Activations of caspase-8 and -3 were detected from dying HAoSMCs. 7-Ketocholesterol inhibited translocation of the nuclear factor κB (NF-κB) subunits of p65 and p50 from the cytosol into the nucleus, increase of NF-κB activity, and expression of caspase-8 homolog Fas ligand interleukin-1-converting enzyme inhibitory protein by TNF-α. We also found that X-chromosome-linked inhibitor of apoptosis protein was degraded in dying HAoSMC. The present study proposes that 7-ketocholesterol would contribute to the disappearance of HVSMC in the atherosclerotic lesions by enhancing receptor-mediated death. This is the first report demonstrating induction of TNF-α-mediated death by oxysterol in cells.-
dc.publisherElsevier-
dc.titleTNF-α activates death pathway in human aorta smooth muscle cell in the presence of 7-ketocholesterol-
dc.title.alternativeTNF-α activates death pathway in human aorta smooth muscle cell in the presence of 7-ketocholesterol-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number4-
dc.citation.endPage1099-
dc.citation.startPage1093-
dc.citation.volume333-
dc.contributor.affiliatedAuthorHyun Sun Lee-
dc.contributor.affiliatedAuthorJong Sun Chang-
dc.contributor.affiliatedAuthorJin Ah Baek-
dc.contributor.affiliatedAuthorMi Yeon Chung-
dc.contributor.affiliatedAuthorMun Chual Rho-
dc.contributor.affiliatedAuthorYoung Kook Kim-
dc.contributor.alternativeName이현선-
dc.contributor.alternativeName장종선-
dc.contributor.alternativeName백진아-
dc.contributor.alternativeName정미연-
dc.contributor.alternativeName이한철-
dc.contributor.alternativeName임병용-
dc.contributor.alternativeName석대은-
dc.contributor.alternativeName노문철-
dc.contributor.alternativeName김영국-
dc.contributor.alternativeName김관회-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 333, no. 4, pp. 1093-1099-
dc.identifier.doi10.1016/j.bbrc.2005.05.196-
dc.subject.keyword7-Ketocholesterol-
dc.subject.keywordatherosclerosis-
dc.subject.keywordTNF-α-
dc.subject.keywordvascular smooth muscle cell-
dc.subject.local7-Ketocholesterol-
dc.subject.localatherosclerosis-
dc.subject.localAtherosclerosis-
dc.subject.localatheroclerosis-
dc.subject.localTumor necrosis fa tor-α-
dc.subject.localTNFα-
dc.subject.localTumor necrosis factor (TNF)-α-
dc.subject.localTnf-α-
dc.subject.localTNF-a-
dc.subject.localTNF-alpha-
dc.subject.localTumor necrosis factor-α-
dc.subject.localtumor necrosis factor-alpha-
dc.subject.localTNFa-
dc.subject.localTumor necrosis factor alpha-
dc.subject.localTumor necrosis factor-alpha-
dc.subject.localTNF-α-
dc.subject.localtumor necrosis factor-α-
dc.subject.localVascular smooth muscle cell-
dc.subject.localvascular smooth muscle cell-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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