Chromosomal integration of sfp gene in Bacillus subtilis to enhance bioavailability of hydrophobic liquids

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Title
Chromosomal integration of sfp gene in Bacillus subtilis to enhance bioavailability of hydrophobic liquids
Author(s)
Young Ki Lee; Seong Bin Kim; Chan Sun Park; J G Kim; Hee-Mock Oh; Byung Dae Yoon; Hee-Sik Kim
Bibliographic Citation
Applied Microbiology and Biotechnology, vol. 67, no. 6, pp. 789-794
Publication Year
2005
Abstract
Bacillus subtilis C9 effectively degrades aliphatic hydrocarbons up to a chain length of C19 and produces a lipopeptide-type biosurfactant, surfactin, yet it has no genetic competency. Therefore, to obtain a transformable surfactin producer, the sfp gene cloned from B. subtilis C9 was integrated into the chromosome of B. subtilis 168, a non-surfactin producer, by homologous recombination. The transformants reduced the surface tension of the culture broth from 70.0 mN/m to 28.0 mN/m, plus the surface-active compound produced by the transformants exhibited the same R f value as that from B. subtilis C9 and authentic surfactin in a thin-layer chromatographic analysis. The integration of the sfp gene into the chromosome of B. subtilis 168 was confirmed by Southern hybridization. Like B. subtilis C9, the transformants readily degraded n-hexadecane, although the original strain did not. It was also statistically confirmed that the hydrocarbon degradation of the transformants was highly correlated to their surfactin production by the determination of the correlation coefficient (r 2=0.997, P<0.01). Therefore, these results indicate that the surfactin produced from B. subtilis enhances the bioavailability of hydrophobic liquids.
ISSN
0175-7598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00253-004-1847-4
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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