Human β-globin second intron highly enhances expression of foreign genes from murine cytomegalovirus immediate-early promoter

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Title
Human β-globin second intron highly enhances expression of foreign genes from murine cytomegalovirus immediate-early promoter
Author(s)
M Kang; Seon-Young Kim; S Lee; Y K Lee; J Lee; H S Shin; Y S Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 15, no. 3, pp. 544-550
Publication Year
2005
Abstract
To develop a highly efficient mammalian expression vector, a series of vectors were constructed based on the murine cytomegalovirus (MCMV) immediate-early (IE) promoter and human β-globin second intron. The resulting MCMV promoter was several-fold stronger than the HCMV promoter in various mammalian cell lines, such as the NIH3T3, Neuro-2a, 293T, and HT1080 cell lines, and was only slightly weaker than the HCMV promoter in HeLa and CHO cells. The inclusion of the human β-globin second intron behind the MCMV promoter or HCMV promoter markedly enhanced the promoter activity in various mammalian cell lines, and the resultant MCMV/Glo-I expression system was stronger than the HCMV promoter from 4.7- to 11.2-fold in every cell line tested. Also, the MCMV/Glo-I promoter induced a higher level of the VSV-G protein in a transiently transfected 293T cell line, which is useful for the production of recombinant retrovirus and lentivirus vectors.
Keyword
high-level gene expressionhuman β-globin second intronMCMV IE promoter
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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