Development of PCR-ELISA for the detection of hepatitis B virus x gene expression and clinical application

Cited 5 time in scopus
Metadata Downloads
Title
Development of PCR-ELISA for the detection of hepatitis B virus x gene expression and clinical application
Author(s)
J W Kim; Jung Hyun Shim; J W Park; W C Jang; H K Chang; I H Song; S Y Baek; S H Lee; Do Young Yoon; S N Park
Bibliographic Citation
Journal of Clinical Laboratory Analysis, vol. 19, no. 4, pp. 139-145
Publication Year
2005
Abstract
The presence of hepatitis B virus x (HBx) antigen/antibody is known to correlate with the well-established serological markers of ongoing viral replication in the chronic phase of HBV infection, and strongly suggests that the level and duration of HBx expression may influence the outcome of the chronic infection. In this research, we developed a polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) method for the detection of HBx gene expression. We also investigated its relationship to the progress of the disease in HBV-related patients. Peripheral blood mononuclear cells (PBMCs) were purely isolated, and reverse transcription-PCR (RT-PCR) was performed for improved sensitivity. The PCR products were determined by ELISA, and we investigated the relationship of the proposed method to the clinical status of the patients. The PCR-ELISA used in this work was found to be at least 100 times more sensitive than the conventional PCR method, and even 8,000-fold diluted PCR products could be detected. The HBx concentrations significantly differed among control subjects (0.36±0.09, [P<0.01] and patients with chronic hepatitis (1.13±0.34 [P<0.01 compared to control]), liver cirrhosis (LC; 1.37±0.28 [P<0.01 compared to control]), and hepatocellular carcinoma (HCC; 1.48±0.95 [P<0.01 compared to control]). These findings suggest that monitoring of HBx could be useful for early diagnosis and prognosis in patients with chronic HBV infection, LC, and HCC.
Keyword
Hepatitis B virus x(HBx)PCR-ELISAPeripheral blood mononuclear cell (PBMC)
ISSN
0887-8013
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/jcla.20068
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.