더덕의 체세포배발생에서 전형층분화와 자엽수의 관계

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Title
더덕의 체세포배발생에서 전형층분화와 자엽수의 관계
Author(s)
P S Choi; W Y Soh; D Y Cho; Jang Ryol Liu
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 32, no. 2, pp. 135-138
Publication Year
2005
Abstract
Embryogenic callus was obtained from cotyledonary explants of Codonopsis lanceloata on Murashige & Skoog's medium supplemented with 1 mg/L 2,4-D. Suspension cultures of the embryogenic calli were grown on a shaker at 100 strokes/min, and then the calli were subcultured for 2 weeks in 2,4-D-free medium to produce somatic embryo. In somatic embryos at the globular stage, cotyledon initials began to differentiate themselves in the near distal end of the procambial strand. Dicotyledons, tricotyledon, tetracotyledon and fused cotyledon were differentiated from the distal ends of two, three, four and circular procambial strands, respectively. Nearly circular procambial strand in lower hypocotyls were independently differentiated into two, three, four procambial tissues at cotyledonary node and cotyledons to form somatic embryos with dicotyledon, tricotyledon, tetracotyledon. If the distal subepidermal cells of globular embryo exclusively became cotyledon initials, the torpedo or cotyledonary embryo was characterized by somatic embryos with fused cotyledon.
Keyword
dicotyledonfused cotyledonontogenysomatic embryotetracotyledontricotyledon
ISSN
1015-5880
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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