Production of transgenic maize (Zea mays L.) using Agrobacterium tumefaciens-mediated transformation = Agrobacterium tumefaciens 공동배양법을 이용한 옥수수 형질전환체 생산

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Title
Production of transgenic maize (Zea mays L.) using Agrobacterium tumefaciens-mediated transformation = Agrobacterium tumefaciens 공동배양법을 이용한 옥수수 형질전환체 생산
Author(s)
M A Cho; Y O Park; J S Kim; K J Park; H K Min; Jang Ryol Liu; T Clemente; P S Choi
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 32, no. 2, pp. 91-95
Publication Year
2005
Abstract
Agrobacterium tumefaciens-mediated immature embryo transformation was used to produce transgenic maize. Immature embryo of Hi II genotype were co-cultivated with strains Agrobacterium tumefaciens (C58C1) containing the binary vectors (pPTN290) carrying with Ubiquitin promoter-GUS gene as reporter gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent. Seven embryogenic callus lines transformed showed the resistance in paromomycin antibiotics. Histochemical GUS assay showed that 7 individual lines transformed with the GUS gene were positive response among the transformants. Southern blot analysis revealed that the nptll gene segregated and expressed in their progeny.
Keyword
agrobacterium strainsβ-glucuroinidase (GUS)maizetransformation
ISSN
1015-5880
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2005.32.2.091
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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