C-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2 = Bcl-2 유사인자 Bfl-1과 결합하는 IKK-β의 C말단 Ser부위

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dc.contributor.authorHyun Kyung Yoon-
dc.contributor.authorJin Koo Kim-
dc.contributor.authorHee Jun Cho-
dc.contributor.authorMi Young Cho-
dc.contributor.authorYuk-Pheel Park-
dc.contributor.authorK H You-
dc.contributor.authorJ W Kim-
dc.contributor.authorDo Young Yoon-
dc.contributor.authorYong Kyung Choe-
dc.contributor.authorHee Gu Lee-
dc.date.accessioned2017-04-19T09:03:07Z-
dc.date.available2017-04-19T09:03:07Z-
dc.date.issued2005-
dc.identifier.issnI000-0170-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7030-
dc.description.abstractBackground : Bcl-2 family proteins play a central role in regulating apoptosis. In human, over 20 members of this family have been identified to date. Bfl-1, a member of the Bcl-2 family, has been known to retard apoptosis in various cell lines. However, the function of Bfl-1 remains unclear. Methods : In order to investigate the Bfl-1 function, we employed yeast two-hybrid system to identify the proteins which are capable of interacting with Bfl-1. The interaction of inhibitor kappaB kinase- (IKK- ) and Bfl-1 was confirmed using glutathione S-transferase pull down assays. To determine which regions of IKK- were required for interaction with Bfl-1, we constructed 12 deletion mutants of IKK- and 5 deletion mutants of Bfl-1. Results : Bfl-1 interacted with the C-terminal region of IKK- which is a subunit of IKK complex, and IKK- activity is very important in the NF- B related pathway. In addition, the amino acids 673-745 of IKK- were important for Bfl-1 interactions, and amino acids 1-484 of Bfl-1, including Bcl-2 homology domains (BH1, BH2, BH3, BH4), were crucial for IKK- interactions. Conclusions : IKK C-terminus contains many serine residues as binding partner of Bfl-1. Our results suggested that Bfl-1 is involved in the NF- B activation through interaction of IKK- and Bfl-1. Further studies need to be performed to understand functions of the IKK- and Bfl-1 associated with the regulation of the NF- B activation pathway.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleC-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2 = Bcl-2 유사인자 Bfl-1과 결합하는 IKK-β의 C말단 Ser부위-
dc.title.alternativeC-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2-
dc.typeArticle-
dc.citation.titleKorean Journal of Laboratory Medicine-
dc.citation.number3-
dc.citation.endPage198-
dc.citation.startPage192-
dc.citation.volume25-
dc.contributor.affiliatedAuthorJin Koo Kim-
dc.contributor.affiliatedAuthorHee Jun Cho-
dc.contributor.affiliatedAuthorMi Young Cho-
dc.contributor.affiliatedAuthorYuk-Pheel Park-
dc.contributor.affiliatedAuthorDo Young Yoon-
dc.contributor.affiliatedAuthorYong Kyung Choe-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName윤현경-
dc.contributor.alternativeName김진구-
dc.contributor.alternativeName조희준-
dc.contributor.alternativeName조미영-
dc.contributor.alternativeName박육필-
dc.contributor.alternativeName유관희-
dc.contributor.alternativeName김종완-
dc.contributor.alternativeName윤도영-
dc.contributor.alternativeName최용경-
dc.contributor.alternativeName이희구-
dc.identifier.bibliographicCitationKorean Journal of Laboratory Medicine, vol. 25, no. 3, pp. 192-198-
dc.subject.keywordBfl-1-
dc.subject.keywordIKK- yeast two hybrid-
dc.subject.keywordBH domain-
dc.subject.localBfl-1-
dc.subject.localIKK- yeast two hybrid-
dc.subject.localBH domain-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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