DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyun Kyung Yoon | - |
dc.contributor.author | Jin Koo Kim | - |
dc.contributor.author | Hee Jun Cho | - |
dc.contributor.author | Mi Young Cho | - |
dc.contributor.author | Yuk-Pheel Park | - |
dc.contributor.author | K H You | - |
dc.contributor.author | J W Kim | - |
dc.contributor.author | Do Young Yoon | - |
dc.contributor.author | Yong Kyung Choe | - |
dc.contributor.author | Hee Gu Lee | - |
dc.date.accessioned | 2017-04-19T09:03:07Z | - |
dc.date.available | 2017-04-19T09:03:07Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | I000-0170 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7030 | - |
dc.description.abstract | Background : Bcl-2 family proteins play a central role in regulating apoptosis. In human, over 20 members of this family have been identified to date. Bfl-1, a member of the Bcl-2 family, has been known to retard apoptosis in various cell lines. However, the function of Bfl-1 remains unclear. Methods : In order to investigate the Bfl-1 function, we employed yeast two-hybrid system to identify the proteins which are capable of interacting with Bfl-1. The interaction of inhibitor kappaB kinase- (IKK- ) and Bfl-1 was confirmed using glutathione S-transferase pull down assays. To determine which regions of IKK- were required for interaction with Bfl-1, we constructed 12 deletion mutants of IKK- and 5 deletion mutants of Bfl-1. Results : Bfl-1 interacted with the C-terminal region of IKK- which is a subunit of IKK complex, and IKK- activity is very important in the NF- B related pathway. In addition, the amino acids 673-745 of IKK- were important for Bfl-1 interactions, and amino acids 1-484 of Bfl-1, including Bcl-2 homology domains (BH1, BH2, BH3, BH4), were crucial for IKK- interactions. Conclusions : IKK C-terminus contains many serine residues as binding partner of Bfl-1. Our results suggested that Bfl-1 is involved in the NF- B activation through interaction of IKK- and Bfl-1. Further studies need to be performed to understand functions of the IKK- and Bfl-1 associated with the regulation of the NF- B activation pathway. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | C-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2 = Bcl-2 유사인자 Bfl-1과 결합하는 IKK-β의 C말단 Ser부위 | - |
dc.title.alternative | C-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2 | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Laboratory Medicine | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 198 | - |
dc.citation.startPage | 192 | - |
dc.citation.volume | 25 | - |
dc.contributor.affiliatedAuthor | Jin Koo Kim | - |
dc.contributor.affiliatedAuthor | Hee Jun Cho | - |
dc.contributor.affiliatedAuthor | Mi Young Cho | - |
dc.contributor.affiliatedAuthor | Yuk-Pheel Park | - |
dc.contributor.affiliatedAuthor | Do Young Yoon | - |
dc.contributor.affiliatedAuthor | Yong Kyung Choe | - |
dc.contributor.affiliatedAuthor | Hee Gu Lee | - |
dc.contributor.alternativeName | 윤현경 | - |
dc.contributor.alternativeName | 김진구 | - |
dc.contributor.alternativeName | 조희준 | - |
dc.contributor.alternativeName | 조미영 | - |
dc.contributor.alternativeName | 박육필 | - |
dc.contributor.alternativeName | 유관희 | - |
dc.contributor.alternativeName | 김종완 | - |
dc.contributor.alternativeName | 윤도영 | - |
dc.contributor.alternativeName | 최용경 | - |
dc.contributor.alternativeName | 이희구 | - |
dc.identifier.bibliographicCitation | Korean Journal of Laboratory Medicine, vol. 25, no. 3, pp. 192-198 | - |
dc.subject.keyword | Bfl-1 | - |
dc.subject.keyword | IKK- yeast two hybrid | - |
dc.subject.keyword | BH domain | - |
dc.subject.local | Bfl-1 | - |
dc.subject.local | IKK- yeast two hybrid | - |
dc.subject.local | BH domain | - |
dc.description.journalClass | Y | - |
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