Vasodilation effect of drypetes rhizoma and it's various fractions on isolated rat thoracic aorta and abdominal aorta = 오수수와 그 분획물이 흰쥐의 흉부대동맥과 복대동맥에 미치는 혈관이완효과

Abstract

Objectives : We have examined the relaxational response to the methanol extract of Osusu in isolated thoracic aorta and abdominal aorta from sprague dawley (SD) rat. Methods : Rat thoracic aorta and abdominal aorta were investigated in vessel segments suspended for isometric tension recording by polygraph. To compare the vasodilation effect, put Osusu extract and fractions into them which vessels were precontracted by 5-hydroxytryptamine (5-HT). Results : We found out this result ; the thoracic aorta segments were responded to Osusu with a dose-dependent and concentration-dependent vasorelaxation. The thoracic aorta and abdominal aorta segments were responded to Osusu with a dose-dependent vasodilation. The 5-HT induced vasocontraction at 10M were vasodilated such as 96.8% in thoracic aorta with 30 mg/ml Osusu (Root). Addition of 10 mg/ml Osusu in thoracic aorta which was precontracted by the 5-HT at 10^M were come out differently 44.5% (fr. 1; 80% methanol fraction), 56.4% (fr. 2; hexane fraction), 7.2% (fr. 3; chloroform fraction), 16.8% (fr. 4; ethyl acetate fraction), 54.9% (fr. 5; butanol fraction) and 18.3% (fr. 6; water fraction). Osusu (1 mg/ml) in abdominal aorta which was precontracted by the 5-HT at 10M were come out differently 87.1% (fr. 1; 80% methanol fracton), 76.9% (fr. 2; hexane fraction), 47.2% (fr. 3; chloroform fraction), 63.2% (fr. 4; ethyl acetate fraction), 25.2% (fr. 5; butanol fraction) and 349% (fr. 6; water fraction). Conclusion : Osusu methanol extract and fractions-induced relaxation in the isolated rat thoracic aorta and abdominal aorta were come out be dose-dependent relaxation.