The hematopoietic transcription factor PU.1 regulates RANK gene expression in myeloid progenitors

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dc.contributor.authorO H Kwon-
dc.contributor.authorC K Lee-
dc.contributor.authorYoung Ik Lee-
dc.contributor.authorS G Paik-
dc.contributor.authorHyun Jun Lee-
dc.date.accessioned2017-04-19T09:03:15Z-
dc.date.available2017-04-19T09:03:15Z-
dc.date.issued2005-
dc.identifier.issn0006291X-
dc.identifier.uri10.1016/j.bbrc.2005.07.092ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7057-
dc.description.abstractOsteoclasts are bone resorbing cells of hematopoietic origin. The hematopoietic transcription factor PU.1 is critical for osteoclastogenesis; however, the molecular mechanisms of PU.1-regulated osteoclastogenesis have not been explored. Here, we present evidence that the receptor activator of nuclear factor κB (RANK) gene that has been shown to be crucial for osteoclastogenesis is a transcriptional target of PU.1. The PU.1-/- progenitor cells failed to express the RANK gene and reconstitution of PU.1 in these cells induced RANK expression. Treatment of the PU.1 reconstituted cells with M-CSF and RANKL further augmented the RANK gene expression. To explore the regulatory mechanism of the RANK gene expression by PU.1, we have cloned the human RANK promoter. Transient transfection assays have revealed that the 2.2-kb RANK promoter was functional in a monocyte line RAW264.7, whereas co-transfection of PU.1 transactivated the RANK promoter in HeLa cells. Taken together, these results suggest that PU.1 regulates the RANK gene transcription and this may represent one of the key roles of PU.1 in osteoclast differentiation.-
dc.publisherElsevier-
dc.titleThe hematopoietic transcription factor PU.1 regulates RANK gene expression in myeloid progenitors-
dc.title.alternativeThe hematopoietic transcription factor PU.1 regulates RANK gene expression in myeloid progenitors-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number2-
dc.citation.endPage446-
dc.citation.startPage437-
dc.citation.volume335-
dc.contributor.affiliatedAuthorHyun Jun Lee-
dc.contributor.alternativeName권오형-
dc.contributor.alternativeName이종길-
dc.contributor.alternativeName이영익-
dc.contributor.alternativeName백상기-
dc.contributor.alternativeName이현준-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 335, no. 2, pp. 437-446-
dc.identifier.doi10.1016/j.bbrc.2005.07.092-
dc.subject.keyworddifferentiation-
dc.subject.keywordhematopoiesis-
dc.subject.keywordM-CSF-
dc.subject.keywordosteoclast-
dc.subject.keywordpromoter-
dc.subject.keywordPU.1-
dc.subject.keywordRANK-
dc.subject.keywordtranscription factor-
dc.subject.localdifferentiation-
dc.subject.localDifferentiation-
dc.subject.localhematopoiesis-
dc.subject.localHematopoiesis-
dc.subject.localM-CSF-
dc.subject.localosteoclast-
dc.subject.localOsteoclast-
dc.subject.localpromoter-
dc.subject.localPromoter-
dc.subject.localPU.1-
dc.subject.localRANK-
dc.subject.localtranscription factor-
dc.subject.localTranscription factor-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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