Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-Xylene

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dc.contributor.authorJ Y Jang-
dc.contributor.authorDockyu Kim-
dc.contributor.authorH W Bae-
dc.contributor.authorK Y Choi-
dc.contributor.authorJ C Chae-
dc.contributor.authorG J Zylstra-
dc.contributor.authorY M Kim-
dc.contributor.authorE Kim-
dc.date.accessioned2017-04-19T09:03:17Z-
dc.date.available2017-04-19T09:03:17Z-
dc.date.issued2005-
dc.identifier.issn1225-8873-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7065-
dc.description.abstractRhodococcus sp. strain YU6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. Unlike most other o-xylene-degrading bacteria, YU6 is able to grow on p-xylene. Numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that YU6 initially metabolizes o- and p-xylene by direct aromatic ring oxidation. This leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage pathway. The gene encoding meta-cleavage dioxygenase enzyme was PCR cloned from genomic YU6 DNA using previously known gene sequence data from the o-xylene-degrading Rhodococcus sp. strain DK17. Subsequent sequencing of the 918-bp PCR product revealed a 98% identity to the gene, encoding methylcatechol 2,3-dioxygenase from DK17. PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJYJ1.-
dc.publisherMicrobiological Society Korea-
dc.titleIsolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-Xylene-
dc.title.alternativeIsolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-Xylene-
dc.typeArticle-
dc.citation.titleJournal of Microbiology-
dc.citation.number4-
dc.citation.endPage330-
dc.citation.startPage325-
dc.citation.volume43-
dc.contributor.affiliatedAuthorDockyu Kim-
dc.contributor.alternativeName장정연-
dc.contributor.alternativeName김덕규-
dc.contributor.alternativeName배현원-
dc.contributor.alternativeName최기영-
dc.contributor.alternativeName채종찬-
dc.contributor.alternativeNameZylstra-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeName김응빈-
dc.identifier.bibliographicCitationJournal of Microbiology, vol. 43, no. 4, pp. 325-330-
dc.subject.keywordcatechol 2,3-dioxygenase-
dc.subject.keywordmegaplasmid-
dc.subject.keywordrhodococcus-
dc.subject.keywordxylene isomers-
dc.subject.localcatechol 2,3-dioxygenase-
dc.subject.localCatechol 2,3-dioxygenase-
dc.subject.localmegaplasmid-
dc.subject.localrhodococcus-
dc.subject.localRhodococcus-
dc.subject.localxylene isomers-
dc.description.journalClassY-
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