DC Field | Value | Language |
---|---|---|
dc.contributor.author | J Y Jang | - |
dc.contributor.author | Dockyu Kim | - |
dc.contributor.author | H W Bae | - |
dc.contributor.author | K Y Choi | - |
dc.contributor.author | J C Chae | - |
dc.contributor.author | G J Zylstra | - |
dc.contributor.author | Y M Kim | - |
dc.contributor.author | E Kim | - |
dc.date.accessioned | 2017-04-19T09:03:17Z | - |
dc.date.available | 2017-04-19T09:03:17Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7065 | - |
dc.description.abstract | Rhodococcus sp. strain YU6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. Unlike most other o-xylene-degrading bacteria, YU6 is able to grow on p-xylene. Numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that YU6 initially metabolizes o- and p-xylene by direct aromatic ring oxidation. This leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage pathway. The gene encoding meta-cleavage dioxygenase enzyme was PCR cloned from genomic YU6 DNA using previously known gene sequence data from the o-xylene-degrading Rhodococcus sp. strain DK17. Subsequent sequencing of the 918-bp PCR product revealed a 98% identity to the gene, encoding methylcatechol 2,3-dioxygenase from DK17. PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJYJ1. | - |
dc.publisher | Microbiological Society Korea | - |
dc.title | Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-Xylene | - |
dc.title.alternative | Isolation and characterization of a Rhodococcus species strain able to grow on ortho- and para-Xylene | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 330 | - |
dc.citation.startPage | 325 | - |
dc.citation.volume | 43 | - |
dc.contributor.affiliatedAuthor | Dockyu Kim | - |
dc.contributor.alternativeName | 장정연 | - |
dc.contributor.alternativeName | 김덕규 | - |
dc.contributor.alternativeName | 배현원 | - |
dc.contributor.alternativeName | 최기영 | - |
dc.contributor.alternativeName | 채종찬 | - |
dc.contributor.alternativeName | Zylstra | - |
dc.contributor.alternativeName | 김영민 | - |
dc.contributor.alternativeName | 김응빈 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology, vol. 43, no. 4, pp. 325-330 | - |
dc.subject.keyword | catechol 2,3-dioxygenase | - |
dc.subject.keyword | megaplasmid | - |
dc.subject.keyword | rhodococcus | - |
dc.subject.keyword | xylene isomers | - |
dc.subject.local | catechol 2,3-dioxygenase | - |
dc.subject.local | Catechol 2,3-dioxygenase | - |
dc.subject.local | megaplasmid | - |
dc.subject.local | rhodococcus | - |
dc.subject.local | Rhodococcus | - |
dc.subject.local | xylene isomers | - |
dc.description.journalClass | Y | - |
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