Silencing of NbNAP1 encoding a plastidic SufB-like protein affects chloroplast development in Nicotiana benthamiana

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dc.contributor.authorC S Ahn-
dc.contributor.authorJeong Hee Lee-
dc.contributor.authorH S Pai-
dc.date.accessioned2017-04-19T09:03:17Z-
dc.date.available2017-04-19T09:03:17Z-
dc.date.issued2005-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7066-
dc.description.abstractIt was previously shown that AtNAP1 is a plastidic SufB protein involved in Fe-S cluster assembly in Arabidopsis. In this study, we investigated the effects of depleting SufB protein from plant cells using virus-induced gene silencing (VIGS). VIGS of NbNAP1 encoding a Nicotiana benthamiana homolog of AtNAP1 resulted in a leaf yellowing phenotype. NbNAP1 was expressed ubiquitously in plant tissues with the highest level in roots. A GFP fusion protein of the N-terminal region (M1-V103) of NbNAP1 was targeted to chloroplasts. Depletion of NbNAP1 resulted in reduced numbers of chloroplasts of reduced size. Mitochondria also seemed to be affected. Despite the reduced number and size of the chloroplasts in the NbNAP1 VIGS lines, the expression of many nuclear genes encoding chloroplast-targeted proteins and chlorophyll biosynthesis genes remained unchanged.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleSilencing of NbNAP1 encoding a plastidic SufB-like protein affects chloroplast development in Nicotiana benthamiana-
dc.title.alternativeSilencing of NbNAP1 encoding a plastidic SufB-like protein affects chloroplast development in Nicotiana benthamiana-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number1-
dc.citation.endPage118-
dc.citation.startPage112-
dc.citation.volume20-
dc.contributor.affiliatedAuthorJeong Hee Lee-
dc.contributor.alternativeName안창숙-
dc.contributor.alternativeName이정희-
dc.contributor.alternativeName배현숙-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 20, no. 1, pp. 112-118-
dc.subject.keywordABC Protein-
dc.subject.keywordchloroplast localization-
dc.subject.keywordFe-S cluster-
dc.subject.keywordNbNAP1-
dc.subject.keywordplastid factor-
dc.subject.keywordvirus-induced gene silencing-
dc.subject.localABC Protein-
dc.subject.localchloroplast localization-
dc.subject.localFe-S cluster-
dc.subject.localNbNAP1-
dc.subject.localplastid factor-
dc.subject.localvirus-induced gene silencing-
dc.subject.localVIGS-
dc.subject.localVirus-induced gene silencing-
dc.subject.localVirus-induced gene silencing (VIGS)-
dc.description.journalClassY-
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