Immuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus = Ininitus obliquus에서 분리한 다당류의 면역증강효과

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Title
Immuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus = Ininitus obliquus에서 분리한 다당류의 면역증강효과
Author(s)
Y O Kim; Sang Bae Han; Hong-Weon Lee; Hyo Jung Ahn; Yeo Dae Yoon; Joon Ki Jung; Hwan Mook Kim; C S Shin
Bibliographic Citation
Life Sciences, vol. 77, no. 19, pp. 2438-2456
Publication Year
2005
Abstract
Inonotus obliquus BELYU1102 was selected from 12 different strains of Inonotus as a producer of immuno-stimulating polysaccharide. After a batch fermentation of I. obliquus BELYU1102 was carried out in a 300 l pilot vessel, endo-polysaccharide and exo-polysaccharide were both obtained. The proliferation activity of endo-polysaccharide for splenic cells was much higher than the activity of exo-polysaccharide. The active endo-polysaccharide was produced primarily during the late stationary phase. Enhanced proliferation and polyclonal IgM antibody production were observed in B cells by purified water-soluble endo-polysaccharide. Nitrite production and expression of IL-1β, IL-6, TNF-α, and iNOS in macrophages were also enhanced. However, the endo-polysaccharide did not affect the proliferation of T cells, the IL-2 expression of Th1 cells, or the IL-4 expression of Th2 cells. The endo-polysaccharide showed activities similar to lipopolysaccharide (LPS) for B cells and macrophages, but there was a large difference between the two polysaccharides because cellular activations induced by endo-polysaccharide were not affected by polymyxin B, a specific inhibitor of LPS. The endo-polysaccharide appeared to have other cellular binding sites with TLR-4 and did not show a direct toxicity against tumor cells. However, indirect anti-cancer effects via immuno-stimulation were observed. The mycelial endo-polysaccharide of I. obliquus is a candidate for use as an immune response modifier. Submerged mycelial cultures are advantageous for industrial production of polysaccharides.
Keyword
Immuno-stimulating activityInonotus obliquusMycelial fermentationWater-soluble endo-polysaccharide
ISSN
0024-3205
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.lfs.2005.02.023
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > 1. Journal Articles
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