Development of molecular identification method for genus Alexandrium (Dinophyceae) using whole-cell FISH

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Title
Development of molecular identification method for genus Alexandrium (Dinophyceae) using whole-cell FISH
Author(s)
Choong-Jae Kim; C H Kim; Y Sako
Bibliographic Citation
Marine Biotechnology, vol. 7, no. 3, pp. 215-222
Publication Year
2005
Abstract
We have developed a method to identify species in the genus Alexandrium using whole-cell fluorescent in situ hybridization with FITC-labeled oligonucleotide probes that target large subunit ribosomal rRNA molecules. The probes were designed based on the sequence of the rDNA D1-D2 region of Alexandrium species. DNA probes specific for toxic A. tamarense and A. catenella and nontoxic A. affine, A. fraterculus, A. insuetum, and A. pseudogonyaulax, respectively, were applied to vegetative cells of all above Alexandrium species to test the sensitivity of the probes. Each DNA probe hybridized specifically with vegetative cells of the corresponding Alexandrium species and showed no cross-reactivity to noncorresponding Alexandrium species. In addition, no cross-reactivity of the probes was observed in experiments using concentrated natural seawater samples. The TAMAD2 probe, which is highly specific to A. tamarense, a common toxic species in Korean coastal waters, provides a simple and reliable molecular tool for identification of toxic Alexandrium species.
Keyword
AlexandriumDNA probeWhole-cell FISH
ISSN
1436-2228
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10126-004-0424-2
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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