Aeromicrobium alkaliterrae sp. nov., isolated from an alkaline soil, and emended description of the genus Aeromicrobium

Abstract

A Gram-positive, aerobic, non-motile bacterial strain, KSL-107T, was isolated from an alkaline soil from Korea and subjected to a polyphasic taxonomic study. Strain KSL-107T grew optimally at 25 °C and pH 7.0-7.5. Strain KSL-107T had a cell-wall peptidoglycan based on LL-2,6-diaminopimelic acid. It contained MK-9(H4) as the predominant menaquinone and C16:0, C16:0 2-OH and 10-methyl C18:0 as the major fatty acids. The DNA G+C content was 71.5 mol%. Comparative 16S rRNA gene sequence analyses showed that strain KSL-107T was phylogenetically affiliated to the genus Aeromicrobium of the family Nocardioidaceae. Similarity values between the 16S rRNA gene sequence of strain KSL-107T and those of type strains of Aeromicrobium species were in the range 97.9-98.2%. The isolate could be distinguished from other Aeromicrobium species by levels of DNA-DNA relatedness and differences in some phenotypic characteristics. On the basis of the data presented, it is suggested that strain KSL-107T (=KCTC 19073T=DSM 16824T) represents a novel Aeromicrobium species, for which the name Aeromicrobium alkaliterrae sp. nov. is proposed. An emended description of the genus Aeromicrobium is also proposed.