Involvement of nonlamellar-prone lipids in the stability increase of human cytochrome P450 1A2 in reconstituted membranes

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dc.contributor.authorT Ahn-
dc.contributor.authorC H Yun-
dc.contributor.authorDoo-Byoung Oh-
dc.date.accessioned2017-04-19T09:03:25Z-
dc.date.available2017-04-19T09:03:25Z-
dc.date.issued2005-
dc.identifier.issn0006-2960-
dc.identifier.uri10.1021/bi050051eko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7111-
dc.description.abstractThe effect of nonlamellar-prone lipids, diacylglycerol (DG) and phosphatidylethanolamine (PE), on the stability of human cytochrome P450 1A2 (CYP1A2) was examined. When 100% phosphatidylcholine (PC) in standard vesicles was gradually replaced with either DG or PE, the stability of CYP1A2 increased; the incubation time-dependent destruction of spectrally detectable P450, decrease of catalytic activity, reduction of intrinsic fluorescence, and increased sensitivity to trypsin digestion were significantly alleviated. The ternary system of PC/PE/DG increased the stability of CYP1 A2 more, even at lower concentrations of each nonlamellar-prone lipid, than that of the binary lipid mixture (PC/nonlamellar lipid). By incorporating the nonlamellar-prone lipids, the CYP1A2-induced increase of the surface pressure of the lipid monolayer was much higher compared to that for 100% PC. Increased surface pressure indicates a deep insertion of the protein into lipid monolayers. Nonlamellar lipids also increased the transition temperature of CYP1A2 in thermal unfolding and reduced the incubation time-dependent detachment of membrane-bound CYP1A2 from vesicles. Taken together, these results suggest that nonlamellar lipids per se and/or the phase properties of the membrane containing these lipids are important in the enhanced stability of CYP1A2 and the concomitant maintenance of catalytic activity of the protein.-
dc.publisherAmer Chem Soc-
dc.titleInvolvement of nonlamellar-prone lipids in the stability increase of human cytochrome P450 1A2 in reconstituted membranes-
dc.title.alternativeInvolvement of nonlamellar-prone lipids in the stability increase of human cytochrome P450 1A2 in reconstituted membranes-
dc.typeArticle-
dc.citation.titleBiochemistry-
dc.citation.number25-
dc.citation.endPage9196-
dc.citation.startPage9188-
dc.citation.volume44-
dc.contributor.affiliatedAuthorDoo-Byoung Oh-
dc.contributor.alternativeName안태호-
dc.contributor.alternativeName윤철호-
dc.contributor.alternativeName오두병-
dc.identifier.bibliographicCitationBiochemistry, vol. 44, no. 25, pp. 9188-9196-
dc.identifier.doi10.1021/bi050051e-
dc.description.journalClassY-
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