Characterization and Xylanase productivity of Streptomyces sp. WL-2 = Xylanase 생산균 Streptomyces sp. WL-2의 특성과 효소 생산성

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Title
Characterization and Xylanase productivity of Streptomyces sp. WL-2 = Xylanase 생산균 Streptomyces sp. WL-2의 특성과 효소 생산성
Author(s)
E H Lee; Chang-Jin Kim; K H Yoon
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 33, no. 3, pp. 178-183
Publication Year
2005
Abstract
A strain WL-2 was isolated from soil as a producer of the extracellular xylanase, which catalyzes the hydrolysis of oat spelt xylan. The strain WL-2 was identified as Streptomyces sp. on the basis of its 16S rRNA sequence, morphology, cultural and physiological properties. The xylanase of culture filtrate was the most active at 60°C and pH 6.0, and retained 90% of its maximum activity at range of pH 4.5-6.5. In order to optimize the culture medium for xylanase production, ingredients of G.S.S medium were replaced by several carbohydrates. The carbohydrates such as α-cellulose, oat spelt xylan and maltose increased dramatically the xylanase productivity of Streptomyces sp. WL-2. The maximum xylanase productivity was reached to 120 U/ml in the modified medium containing 1% α-cellulose and 1% maltose.
Keyword
streptomycesidentificationxylanaseproductivity
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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