Investigation of possible horizontal gene transfer from the leaf tissue of transgenic potato to soil vacteria

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dc.contributor.authorYoung Tae Kim-
dc.contributor.authorSung Eun Kim-
dc.contributor.authorKi Duk Park-
dc.contributor.authorTae Hoon Kang-
dc.contributor.authorYun Mi Lee-
dc.contributor.authorS H Lee-
dc.contributor.authorJae Sun Moon-
dc.contributor.authorSung Uk Kim-
dc.date.accessioned2017-04-19T09:03:32Z-
dc.date.available2017-04-19T09:03:32Z-
dc.date.issued2005-
dc.identifier.issn10177825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7133-
dc.description.abstractTo monitor the possibility of horizontal gene transfer between transgenic potato and bacteria in the environment, the gene flow from glufosinate-tolerant potato to bacteria in soils was investigated. The soil samples treated with the leaf tissue of either glufosinate-tolerant or glufosinate-sensitive potato were subjected to PCR and Southern hybridization to determine possible occurrence of glufosinate-resistant soil bacteria and to detect the bar (phosphinothricin acetyltransferase) gene, conferring tolerance to glufosinate. The bar gene was not detected from genomic DNAs extracted at different time intervals from the soil samples, which had been treated with the leaf tissue of either transgenic or nontransgenic potato for 2 to 8 weeks. In addition, the level of glufosinate-resistant bacteria isolated from the soil samples treated with the leaf tissue of transgenic potato was similar to that of the samples treated with non-transgenic potato after 4 months of incubation at 25°C. The bar gene was not detected in the genomic DNAs extracted from colonies growing on the plate containing glufosinate, indicating that the bacteria could acquire the resistant phenotype to glufosinate by another mechanism without the uptake of the bar gene from glufosinate-tolerant potato.-
dc.publisherSouth Korea-
dc.titleInvestigation of possible horizontal gene transfer from the leaf tissue of transgenic potato to soil vacteria-
dc.title.alternativeInvestigation of possible horizontal gene transfer from the leaf tissue of transgenic potato to soil vacteria-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number5-
dc.citation.endPage1134-
dc.citation.startPage1130-
dc.citation.volume15-
dc.contributor.affiliatedAuthorJae Sun Moon-
dc.contributor.alternativeName김영태-
dc.contributor.alternativeName김성은-
dc.contributor.alternativeName박기덕-
dc.contributor.alternativeName강태훈-
dc.contributor.alternativeName이윤미-
dc.contributor.alternativeName이상한-
dc.contributor.alternativeName문제선-
dc.contributor.alternativeName김성욱-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 15, no. 5, pp. 1130-1134-
dc.subject.keywordenvironmental risk assessment-
dc.subject.keywordglufosinate-
dc.subject.keywordherbicide tolerance-
dc.subject.keywordhorizontal gene transfer-
dc.subject.keywordmicrocosm-
dc.subject.keywordtransgenic potato-
dc.subject.localenvironmental risk assessment-
dc.subject.localEnvironmental risk assessment-
dc.subject.localglufosinate-
dc.subject.localGlufosinate-
dc.subject.localherbicide tolerance-
dc.subject.localHerbicide tolerance-
dc.subject.localhorizontal gene transfer-
dc.subject.localHorizontal gene transfer-
dc.subject.localmicrocosm-
dc.subject.localtransgenic potato-
dc.subject.localTransgenic potato-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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