DC Field | Value | Language |
---|---|---|
dc.contributor.author | Young Tae Kim | - |
dc.contributor.author | Sung Eun Kim | - |
dc.contributor.author | Ki Duk Park | - |
dc.contributor.author | Tae Hoon Kang | - |
dc.contributor.author | Yun Mi Lee | - |
dc.contributor.author | S H Lee | - |
dc.contributor.author | Jae Sun Moon | - |
dc.contributor.author | Sung Uk Kim | - |
dc.date.accessioned | 2017-04-19T09:03:32Z | - |
dc.date.available | 2017-04-19T09:03:32Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7133 | - |
dc.description.abstract | To monitor the possibility of horizontal gene transfer between transgenic potato and bacteria in the environment, the gene flow from glufosinate-tolerant potato to bacteria in soils was investigated. The soil samples treated with the leaf tissue of either glufosinate-tolerant or glufosinate-sensitive potato were subjected to PCR and Southern hybridization to determine possible occurrence of glufosinate-resistant soil bacteria and to detect the bar (phosphinothricin acetyltransferase) gene, conferring tolerance to glufosinate. The bar gene was not detected from genomic DNAs extracted at different time intervals from the soil samples, which had been treated with the leaf tissue of either transgenic or nontransgenic potato for 2 to 8 weeks. In addition, the level of glufosinate-resistant bacteria isolated from the soil samples treated with the leaf tissue of transgenic potato was similar to that of the samples treated with non-transgenic potato after 4 months of incubation at 25°C. The bar gene was not detected in the genomic DNAs extracted from colonies growing on the plate containing glufosinate, indicating that the bacteria could acquire the resistant phenotype to glufosinate by another mechanism without the uptake of the bar gene from glufosinate-tolerant potato. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Investigation of possible horizontal gene transfer from the leaf tissue of transgenic potato to soil vacteria | - |
dc.title.alternative | Investigation of possible horizontal gene transfer from the leaf tissue of transgenic potato to soil vacteria | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 1134 | - |
dc.citation.startPage | 1130 | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Young Tae Kim | - |
dc.contributor.affiliatedAuthor | Sung Eun Kim | - |
dc.contributor.affiliatedAuthor | Ki Duk Park | - |
dc.contributor.affiliatedAuthor | Tae Hoon Kang | - |
dc.contributor.affiliatedAuthor | Yun Mi Lee | - |
dc.contributor.affiliatedAuthor | Jae Sun Moon | - |
dc.contributor.affiliatedAuthor | Sung Uk Kim | - |
dc.contributor.alternativeName | 김영태 | - |
dc.contributor.alternativeName | 김성은 | - |
dc.contributor.alternativeName | 박기덕 | - |
dc.contributor.alternativeName | 강태훈 | - |
dc.contributor.alternativeName | 이윤미 | - |
dc.contributor.alternativeName | 이상한 | - |
dc.contributor.alternativeName | 문제선 | - |
dc.contributor.alternativeName | 김성욱 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 15, no. 5, pp. 1130-1134 | - |
dc.subject.keyword | environmental risk assessment | - |
dc.subject.keyword | glufosinate | - |
dc.subject.keyword | herbicide tolerance | - |
dc.subject.keyword | horizontal gene transfer | - |
dc.subject.keyword | microcosm | - |
dc.subject.keyword | transgenic potato | - |
dc.subject.local | Environmental risk assessment | - |
dc.subject.local | Environmental Risk assessment | - |
dc.subject.local | Environmental Risk Assessment | - |
dc.subject.local | environmental risk assessment | - |
dc.subject.local | Glufosinate | - |
dc.subject.local | glufosinate | - |
dc.subject.local | herbicide tolerance | - |
dc.subject.local | Herbicide tolerance | - |
dc.subject.local | horizontal gene transfer | - |
dc.subject.local | Horizontal gene transfer | - |
dc.subject.local | microcosm | - |
dc.subject.local | Transgenic potato | - |
dc.subject.local | transgenic potato | - |
dc.description.journalClass | Y | - |
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