DC Field | Value | Language |
---|---|---|
dc.contributor.author | M G Netea | - |
dc.contributor.author | T Azam | - |
dc.contributor.author | G Ferwerda | - |
dc.contributor.author | S E Girardin | - |
dc.contributor.author | M Walsh | - |
dc.contributor.author | J S Park | - |
dc.contributor.author | E Abraham | - |
dc.contributor.author | J M Kim | - |
dc.contributor.author | Do Young Yoon | - |
dc.contributor.author | C A Dinarello | - |
dc.contributor.author | S H Kim | - |
dc.date.accessioned | 2017-04-19T09:03:33Z | - |
dc.date.available | 2017-04-19T09:03:33Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | 10.1073/pnas.0508237102 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7137 | - |
dc.description.abstract | The activation of innate immunity requires the amplification of signals induced by pattern-recognition receptors for bacterial products. We have investigated the role of the newly described cytokine IL-32 in the amplification of cytokine production induced by the two most clinically relevant families of microbial receptors, the cell-surface Toll-like receptors (TLRs) and the intracellular nuclear oligomerization domain (NOD) receptor family. IL-32 synergized with the NOD1- and NOD2-specific muropeptides of peptidoglycans for the release of IL-1β and IL-6 (a 3- to 10-fold increase). In contrast, IL-32 did not influence the cytokine production induced via TLRs. The synergistic effect of IL-32 and synthetic muramyl dipeptide (MDP) on cytokine production was absent in the cells of patients with Crohn's disease bearing the NOD2 frameshift mutation 3020insC, demonstrating that the IL-32/MDP synergism depends on NOD2. This in vitro synergism between IL-32 and NOD2 ligands was consistent with a marked constitutive expression of IL-32 in human colon epithelial tissue. In addition, the potentiating effect of IL-32 on the cytokine production induced by the synthetic muropeptide FK-156 was absent in NOD1-deficient macrophages, supporting the interaction between IL-32 and NOD1 pathways. When specific caspase inhibitors were used, the synergism between IL-32 and MDP/NOD2 depended on the activation of caspase 1. Only additive effects of IL-32 and muropeptides were observed for TNF-α production. The modulation of intracellular NOD2 pathways by IL-32, but not cell-surface TLRs, and the marked expression of IL-32 in colon mucosa suggest a role of IL-32 in the pathogenesis of Crohn's disease. | - |
dc.publisher | Natl Acad Sciences | - |
dc.title | IL-32 synergizes with nucleotide oligomerization domain (NOD) 1 and NOD2 ligands for IL-1β and IL-6 production through a caspase 1-dependent mechanism | - |
dc.title.alternative | IL-32 synergizes with nucleotide oligomerization domain (NOD) 1 and NOD2 ligands for IL-1β and IL-6 production through a caspase 1-dependent mechanism | - |
dc.type | Article | - |
dc.citation.title | Proceedings of National Academy of Sciences of United States of America | - |
dc.citation.number | 45 | - |
dc.citation.endPage | 16314 | - |
dc.citation.startPage | 16309 | - |
dc.citation.volume | 102 | - |
dc.contributor.affiliatedAuthor | Do Young Yoon | - |
dc.contributor.alternativeName | Netea | - |
dc.contributor.alternativeName | Azam | - |
dc.contributor.alternativeName | Ferwerda | - |
dc.contributor.alternativeName | Girardin | - |
dc.contributor.alternativeName | Walsh | - |
dc.contributor.alternativeName | 박종성 | - |
dc.contributor.alternativeName | Abraham | - |
dc.contributor.alternativeName | 김진만 | - |
dc.contributor.alternativeName | 윤도영 | - |
dc.contributor.alternativeName | Dinarello | - |
dc.contributor.alternativeName | 김수현 | - |
dc.identifier.bibliographicCitation | Proceedings of National Academy of Sciences of United States of America, vol. 102, no. 45, pp. 16309-16314 | - |
dc.identifier.doi | 10.1073/pnas.0508237102 | - |
dc.subject.keyword | Cytokines | - |
dc.subject.keyword | Toll-like receptors | - |
dc.subject.local | cytokine | - |
dc.subject.local | Cytokines | - |
dc.subject.local | Cytokine | - |
dc.subject.local | toll-like receptors | - |
dc.subject.local | Toll-like receptor (TLR) | - |
dc.subject.local | Toll-like receptors | - |
dc.subject.local | toll-like receptor | - |
dc.subject.local | TLR | - |
dc.subject.local | toll-like receptor (TLR) | - |
dc.subject.local | Toll-like receptor | - |
dc.description.journalClass | Y | - |
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