Inactivation of organellar glutamyl- and seryl-tRNA synthetases leads to developmental arrest of chloroplasts and mitochondria in higher plants

Cited 31 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorYu Kyung Kim-
dc.contributor.authorJ Y Lee-
dc.contributor.authorHye Sun Cho-
dc.contributor.authorSang Sook Lee-
dc.contributor.authorH J Ha-
dc.contributor.authorS Kim-
dc.contributor.authorDoil Choi-
dc.contributor.authorH S Pai-
dc.date.accessioned2017-04-19T09:03:35Z-
dc.date.available2017-04-19T09:03:35Z-
dc.date.issued2005-
dc.identifier.issn0021-9258-
dc.identifier.uri10.1074/jbc.M504805200ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7157-
dc.description.abstractAminoacyl-tRNA synthetases (ARSs) are key enzymes involved in protein translation, and both cytosolic and organellar forms are present in the genomes of eukaryotes. In this study, we investigated cellular effects of depletion of organellar forms of ARS using virus-induced gene silencing (VIGS) in Nicotiana benthamiana. VIGS of NbERS and NbSRS, which encode organellar GluRS and SerRS, respectively, resulted in a severe leaf-yellowing phenotype. The NbERS and NbSRS genes were ubiquitously expressed in plant tissues, and induced in response to light. Green fluorescent protein (GFP) fusion proteins of the full-length glutamyl-tRNA synthetase (ERS) and seryl-tRNA synthetase (SRS) of Arabidopsis and GFP fusions to the N-terminal extension of these proteins were all dual-targeted to chloroplasts and mitochondria. At the cell level, depletion of NbERS and NbSRS resulted in dramatically reduced numbers of chloroplasts with reduced sizes and chlorophyll content. The numbers and/or physiology of mitochondria were also severely affected. The abnormal chloroplasts lacked most of the thylakoid membranes and appeared to be degenerating, whereas some of them showed doublet morphology, indicating defective chloroplast division. Pulse-field gel electrophoresis analyses demonstrated that chloroplast DNA in subgenomic sizes is the predominant form in the abnormal chloroplasts. Interestingly, despite severe abnormalities in chloroplasts and mitochondria, expression of many nuclear genes encoding chloroplast- or mitochondria-targeted proteins, and chlorophyll biosynthesis genes remained unchanged in the ERS and SRS VIGS lines. This is the first report to analyze the effect of ARS disruption on organelle development in plants.-
dc.publisherAmer Soc Biochemistry Molecular Biology Inc-
dc.titleInactivation of organellar glutamyl- and seryl-tRNA synthetases leads to developmental arrest of chloroplasts and mitochondria in higher plants-
dc.title.alternativeInactivation of organellar glutamyl- and seryl-tRNA synthetases leads to developmental arrest of chloroplasts and mitochondria in higher plants-
dc.typeArticle-
dc.citation.titleJournal of Biological Chemistry-
dc.citation.number44-
dc.citation.endPage37106-
dc.citation.startPage37098-
dc.citation.volume280-
dc.contributor.affiliatedAuthorYu Kyung Kim-
dc.contributor.affiliatedAuthorHye Sun Cho-
dc.contributor.affiliatedAuthorSang Sook Lee-
dc.contributor.affiliatedAuthorDoil Choi-
dc.contributor.alternativeName김유경-
dc.contributor.alternativeName이재용-
dc.contributor.alternativeName조혜선-
dc.contributor.alternativeName이상숙-
dc.contributor.alternativeName하현정-
dc.contributor.alternativeName김성훈-
dc.contributor.alternativeName최도일-
dc.contributor.alternativeName배현숙-
dc.identifier.bibliographicCitationJournal of Biological Chemistry, vol. 280, no. 44, pp. 37098-37106-
dc.identifier.doi10.1074/jbc.M504805200-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.