Immunization with major outer membrane protein of Vibrio vulnificus elicits protective antibodies in a murine model

Cited 0 time in scopus
Metadata Downloads
Title
Immunization with major outer membrane protein of Vibrio vulnificus elicits protective antibodies in a murine model
Author(s)
Cho Rok Jung; M J Park; M S Heo
Bibliographic Citation
Journal of Microbiology, vol. 43, no. 5, pp. 437-442
Publication Year
2005
Abstract
Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on 13% SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG, which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.
Keyword
immunogenecityouter membrane proteinvaccinevibrio vulnificus
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.