The use of glufosinate as a selective marker for the transformation of cucumber (Cucumis sativus L.) = 오이의 형질전환을 위한 선발마커로서 glufosinate의 이용

Cited 0 time in scopus
Metadata Downloads
Title
The use of glufosinate as a selective marker for the transformation of cucumber (Cucumis sativus L.) = 오이의 형질전환을 위한 선발마커로서 glufosinate의 이용
Author(s)
M A Cho; Y M Song; Y O Park; S M Ko; Sung Ran Min; Jang Ryol Liu; P S Choi
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 32, no. 3, pp. 161-165
Publication Year
2005
Abstract
Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.
Keyword
agrobacterium strainsβ-glucuronidase (GUS)leaf painting assaytransgenic cucumberagrobacterium strains
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2005.32.3.161
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.