High frequency plant regeneration from leaf explant cultures of domestic cultivated strawberry (Fragaria x ananassa duch) = 국내 딸기 재배품종의 잎절편 배양으로부터 고빈도 식물체 재생

Abstract

To develop a high efficiency plant regeneration system for in vitro cultures of strawberry, cv. Yeobong, petiole and leaf explants were cultured on MS basal medium containing a combination of 0.5 mg/L IBA and 3.2 mg/L kinetin or zeatin or benzyl amino purine (BAP) for 6 weeks, and leaf explants with dark pretreatment for a week (T₁), 2 weeks (T₂), and 4 weeks (T₃) were cultured on medium supplemented with 0.5 mg/L IBA and 3.2 mg/L zeatin under 16 hr photoperiod for 6 weeks.