Transgenic siberian ginseng cultured cells that produce high levels of human lactoferrin = 인체 락토페린 생산 형질전화 가시오갈피 배양세포

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Title
Transgenic siberian ginseng cultured cells that produce high levels of human lactoferrin = 인체 락토페린 생산 형질전화 가시오갈피 배양세포
Author(s)
Seung Hyun Jo; Suk Yoon Kwon; J W Kim; K T Lee; Sang Soo Kwak; Haeng Soon Lee
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 32, no. 3, pp. 209-215
Publication Year
2005
Abstract
Lactoferrin is an iron-binding glycoprotein with many biological roles, including the protection against microbial and virus infection, stimulation of the immune system. We developed the transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing the human lactoferrin (hLf) protein following Agrobacterium tumefaciens-mediated transformation. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to hLf cDNA under the control of an oxidative stress-inducible SWPA2 promoter was engineered. Transgenic Siberian ginseng cultured cells to produce a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analysis. ELISA and western blot analysis showed that full length-hLf protein was synthesized in the transgenic cells. The production of hLf increased proportionally to cell growth and reached a maximal (up to 3% of total soluble proteins) at the stationary phase. These results suggest that the transgenic Siberian ginseng cultured cells in this study will be biotechnologically useful for the commercial production of medicinal plant cell cultures to produce hLf protein.
Keyword
acanthopanax senticosushigh expression promoterhuman lactoferrinrecombinant proteintransgenic cell cultures
ISSN
1015-5880
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2005.32.3.209
Type
Article
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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