Detection of Bax protein conformational change using a surface plasmon resonance imaging-based antibody chip = SPR 이미징 기반 항체칩을 이용한 Bax 단백질의 구조변화 측정

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Title
Detection of Bax protein conformational change using a surface plasmon resonance imaging-based antibody chip = SPR 이미징 기반 항체칩을 이용한 Bax 단백질의 구조변화 측정
Author(s)
Moonil Kim; Sun Ok Jung; Kyoungsook Park; Eun Ju Jeong; Hyou-Arm Joung; T H Kim; D W Seol; Bong Hyun Chung
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 338, no. 4, pp. 1834-1838
Publication Year
2005
Abstract
We describe an antibody chip technology that uses a surface plasmon resonance (SPR) imaging system to examine the conformational change of a protein. In this study, we used Bax protein, a pro-apoptotic member of the Bcl-2 family of proteins, as a model protein to investigate the conformational alteration triggered by a TNF-related apoptosis-inducing ligand (TRAIL), a potent inducer of apoptosis. To develop the antibody chip for detecting the Bax conformational change, we immobilized Bax monoclonal antibody 6A7, which recognizes only a conformationally changed Bax protein on a gold surface. The resultant immobilized Bax antibodies provided specific and accurate measurements of the active conformation-specific epitope in the apoptotic cancer cells treated with the TRAIL; these measurements corresponded to the data obtained by immunoprecipitation analysis using an active conformation-specific Bax antibody (6A7). The results of our study indicated that TRAIL-induced Bax structural change could be monitored quickly and simply using an SPR imaging system, thus demonstrating the potential for using such a system for the analysis of conformational properties of target proteins.
Keyword
BaxConformational changeSPR imagingSPR sensorTRAIL
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2005.10.155
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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