cDNA microarray analysis of nerve growth factor-regulated gene expression profile in rat PC12 cells

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dc.contributor.authorK H Lee-
dc.contributor.authorChun Jeih Ryu-
dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorJ Kim-
dc.contributor.authorE H Lee-
dc.date.accessioned2017-04-19T09:03:38Z-
dc.date.available2017-04-19T09:03:38Z-
dc.date.issued2005-
dc.identifier.issn0364-3190-
dc.identifier.uri10.1007/s11064-005-2688-yko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7175-
dc.description.abstractNerve growth factor (NGF)-driven differentiation of PC12 cells into neuronal-like cells provides a representative model system for studying neuronal differentiation processes. Despite of extensive research, gene regulation associated with the differentiation program in PC12 cells still needs to be elucidated. We used cDNA microarray analysis to characterize the response of PC12 cells to NGF at mRNA expression. Forty-six genes were reproducibly influenced by 2-fold or more after NGF treatment for 5 days. Twenty-five of the regulated transcripts were matched to genes which have known functions. Among the microarray results confirmed with real-time reverse transcriptase assay, several genes have not previously known to be modulated by NGF. The results mostly reflected changes in molecules regulating neural plasticity, cytoskeletal organization, and lipid metabolism, which include neuritin, PDZ protein Mrt1, lipoprotein lipase, tropomodulin 1 and rhoB. These observed genetic changes may provide new information about molecular mechanisms underlying NGF-promoted differentiation of PC12 cells.-
dc.publisherSpringer-
dc.titlecDNA microarray analysis of nerve growth factor-regulated gene expression profile in rat PC12 cells-
dc.title.alternativecDNA microarray analysis of nerve growth factor-regulated gene expression profile in rat PC12 cells-
dc.typeArticle-
dc.citation.titleNeurochemical Research-
dc.citation.number4-
dc.citation.endPage540-
dc.citation.startPage533-
dc.citation.volume30-
dc.contributor.affiliatedAuthorChun Jeih Ryu-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.alternativeName이경희-
dc.contributor.alternativeName류춘제-
dc.contributor.alternativeName홍효정-
dc.contributor.alternativeName김지영-
dc.contributor.alternativeName이은주-
dc.identifier.bibliographicCitationNeurochemical Research, vol. 30, no. 4, pp. 533-540-
dc.identifier.doi10.1007/s11064-005-2688-y-
dc.subject.keywordcDNA microarray-
dc.subject.keywordGene expression-
dc.subject.keywordNeuronal differentiation-
dc.subject.keywordNGF-
dc.subject.keywordPC12-
dc.subject.localcDNA micro-array-
dc.subject.localcDNA microarray-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localNeuronal differentiation-
dc.subject.localneuronal differentiation-
dc.subject.localNGF-
dc.subject.localPC12-
dc.description.journalClassY-
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