Nerve growth factor down-regulates the expression of chemokines receptors in rat PC12 cells

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dc.contributor.authorK H Lee-
dc.contributor.authorE J H Lee-
dc.contributor.authorH S Park-
dc.contributor.authorChun Jeih Ryu-
dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorJ Y Kim-
dc.description.abstractFunctional expression of norepinephrine transporter (NET) and its regulation were examined in rat pheochromocytoma cell line, PC12. Nerve growth factor (NGF) decreased [3H]-norepinephrine (NE) uptake in association with a decrease in NET mRNA levels. On the other hand, levels of tyrosine hydroxylase mRNA increased in PC12 cells treated with NGF for 4-24 h, while Oct-2 mRNA levels decreased at 4 h with NGF then recovered for 8-24 h in the presence of NGF. Both bFGF and EGF reduced [3H]NE uptake, although they failed to affect NET mRNA levels. To examine the NET transcriptional regulation, we identified the 5'-noncoding region of rat NET mRNA by the rapid amplification of cDNA end (RACE) method and cloned the 5'-flanking region of NET gene. The newly identified exon encodes the untranslated region of rat NET mRNA upstream of the known 5'-region including ATG start codon. Constructs having green fluorescent protein (GFP) as reporter were made with the cloned NET gene, and promoter activity was examined in CHO and SK-N-SH cells transiently transfected and in PC12 cells stably transfected with NET-GFP constructs. The results indicate that the 2.1 kb NET flanking region displays promoter activity and is responsible for the NGF-induced down-regulation of NET expression.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleNerve growth factor down-regulates the expression of chemokines receptors in rat PC12 cells-
dc.title.alternativeNerve growth factor down-regulates the expression of chemokines receptors in rat PC12 cells-
dc.citation.titleExperimental Neurobiology-
dc.contributor.affiliatedAuthorChun Jeih Ryu-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.identifier.bibliographicCitationExperimental Neurobiology, vol. 13, no. 2, pp. 85-90-
dc.subject.keywordnerve growth factor-
dc.subject.keywordchemokine receptors-
dc.subject.keywordmRNA expression-
dc.subject.keywordreal-time PCR-
dc.subject.keywordPC12 cells-
dc.subject.localnerve growth factor-
dc.subject.localchemokine receptors-
dc.subject.localmRNA expression-
dc.subject.localReal-time PCR-
dc.subject.localreal time PCR-
dc.subject.localReal time PCR-
dc.subject.localreal-time PCR-
dc.subject.localPC12 cells-
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