Surface plasmon resonance imaging-based protein arrays for high-throughput screening of protein-protein interaction inhibitors

Cited 62 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSun Ok Jung-
dc.contributor.authorH S Ro-
dc.contributor.authorByung Hoon Kho-
dc.contributor.authorYong Beom Shin-
dc.contributor.authorMin-Gon Kim-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:03:39Z-
dc.date.available2017-04-19T09:03:39Z-
dc.date.issued2005-
dc.identifier.issn1615-9853-
dc.identifier.uri10.1002/pmic.200500001ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7179-
dc.description.abstractThe E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was developed for the high-throughput screening of inhibitor molecules targeting RB-E7 interaction. The glutathione S-transferase-fused E7 protein (GST-E7) was first layered onto a glutathionylated gold chip surface that had been designed to specifically bind to GST-fused proteins. Subsequently, a microarrayer was used to spot the hexa-histidine-tagged RB proteins (His 6-RB) onto the GST-E7-layered gold chip surface, and the resulting SPR image was analyzed. Upon increased His6-RB concentration in the spotting solution, the SPR signal intensity increased proportionally, indicating that His6-RB bound to GST-E7 in a concentration-dependent manner. The His6-RB/GST-E7 interaction was challenged by spotting the His6-RB solution in the presence of a RB binding peptide (PepC) derived from a motif on E7. The SPR imaging data showed that PepC inhibited the His6-RB/GST-E7 interaction in a concentration-dependent manner. Our results show that the SPR imaging-based protein array chip can be applied to screen small molecule inhibitors that target protein-protein interaction.-
dc.publisherWiley-
dc.titleSurface plasmon resonance imaging-based protein arrays for high-throughput screening of protein-protein interaction inhibitors-
dc.title.alternativeSurface plasmon resonance imaging-based protein arrays for high-throughput screening of protein-protein interaction inhibitors-
dc.typeArticle-
dc.citation.titleProteomics-
dc.citation.number17-
dc.citation.endPage4431-
dc.citation.startPage4427-
dc.citation.volume5-
dc.contributor.affiliatedAuthorSun Ok Jung-
dc.contributor.affiliatedAuthorByung Hoon Kho-
dc.contributor.affiliatedAuthorYong Beom Shin-
dc.contributor.affiliatedAuthorMin-Gon Kim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName정선옥-
dc.contributor.alternativeName노현수-
dc.contributor.alternativeName고병훈-
dc.contributor.alternativeName신용범-
dc.contributor.alternativeName김민곤-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationProteomics, vol. 5, no. 17, pp. 4427-4431-
dc.identifier.doi10.1002/pmic.200500001-
dc.subject.keywordhigh-throughput screening-
dc.subject.keywordprotein arrays-
dc.subject.keywordprotein-protein interaction-
dc.subject.keywordsurface plasmon resonance (SPR) imaging-
dc.subject.localhigh-throughput screening-
dc.subject.localHigh-throughput screening-
dc.subject.localHigh-throughput screening (HTS)-
dc.subject.localhighthroughput screening-
dc.subject.localProtein arrays-
dc.subject.localprotein arrays-
dc.subject.localProtein-protein interaction-
dc.subject.localProteinprotein interactions-
dc.subject.localProtein-Protein Interaction-
dc.subject.localProtein-Protein interaction-
dc.subject.localprotein-protein interaction-
dc.subject.localProtein-protein interactions-
dc.subject.localSurface plasmon resonance imaging (SPRI)-
dc.subject.localsurface plasmon resonance imaging-
dc.subject.localSurface plasmon resonance (SPR) imaging-
dc.subject.localsurface plasmon resonance imaging (SPRI)-
dc.subject.localSurface plasmon resonance imaging-
dc.subject.localsurface plasmon resonance (SPR) imaging-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.