DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sun Ok Jung | - |
dc.contributor.author | H S Ro | - |
dc.contributor.author | Byung Hoon Kho | - |
dc.contributor.author | Yong Beom Shin | - |
dc.contributor.author | Min-Gon Kim | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.date.accessioned | 2017-04-19T09:03:39Z | - |
dc.date.available | 2017-04-19T09:03:39Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.uri | 10.1002/pmic.200500001 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7179 | - |
dc.description.abstract | The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was developed for the high-throughput screening of inhibitor molecules targeting RB-E7 interaction. The glutathione S-transferase-fused E7 protein (GST-E7) was first layered onto a glutathionylated gold chip surface that had been designed to specifically bind to GST-fused proteins. Subsequently, a microarrayer was used to spot the hexa-histidine-tagged RB proteins (His 6-RB) onto the GST-E7-layered gold chip surface, and the resulting SPR image was analyzed. Upon increased His6-RB concentration in the spotting solution, the SPR signal intensity increased proportionally, indicating that His6-RB bound to GST-E7 in a concentration-dependent manner. The His6-RB/GST-E7 interaction was challenged by spotting the His6-RB solution in the presence of a RB binding peptide (PepC) derived from a motif on E7. The SPR imaging data showed that PepC inhibited the His6-RB/GST-E7 interaction in a concentration-dependent manner. Our results show that the SPR imaging-based protein array chip can be applied to screen small molecule inhibitors that target protein-protein interaction. | - |
dc.publisher | Wiley | - |
dc.title | Surface plasmon resonance imaging-based protein arrays for high-throughput screening of protein-protein interaction inhibitors | - |
dc.title.alternative | Surface plasmon resonance imaging-based protein arrays for high-throughput screening of protein-protein interaction inhibitors | - |
dc.type | Article | - |
dc.citation.title | Proteomics | - |
dc.citation.number | 17 | - |
dc.citation.endPage | 4431 | - |
dc.citation.startPage | 4427 | - |
dc.citation.volume | 5 | - |
dc.contributor.affiliatedAuthor | Sun Ok Jung | - |
dc.contributor.affiliatedAuthor | Byung Hoon Kho | - |
dc.contributor.affiliatedAuthor | Yong Beom Shin | - |
dc.contributor.affiliatedAuthor | Min-Gon Kim | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 정선옥 | - |
dc.contributor.alternativeName | 노현수 | - |
dc.contributor.alternativeName | 고병훈 | - |
dc.contributor.alternativeName | 신용범 | - |
dc.contributor.alternativeName | 김민곤 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.identifier.bibliographicCitation | Proteomics, vol. 5, no. 17, pp. 4427-4431 | - |
dc.identifier.doi | 10.1002/pmic.200500001 | - |
dc.subject.keyword | high-throughput screening | - |
dc.subject.keyword | protein arrays | - |
dc.subject.keyword | protein-protein interaction | - |
dc.subject.keyword | surface plasmon resonance (SPR) imaging | - |
dc.subject.local | high-throughput screening | - |
dc.subject.local | High-throughput screening | - |
dc.subject.local | High-throughput screening (HTS) | - |
dc.subject.local | highthroughput screening | - |
dc.subject.local | Protein arrays | - |
dc.subject.local | protein arrays | - |
dc.subject.local | Protein-protein interaction | - |
dc.subject.local | Proteinprotein interactions | - |
dc.subject.local | Protein-Protein Interaction | - |
dc.subject.local | Protein-Protein interaction | - |
dc.subject.local | protein-protein interaction | - |
dc.subject.local | Protein-protein interactions | - |
dc.subject.local | Surface plasmon resonance imaging (SPRI) | - |
dc.subject.local | surface plasmon resonance imaging | - |
dc.subject.local | Surface plasmon resonance (SPR) imaging | - |
dc.subject.local | surface plasmon resonance imaging (SPRI) | - |
dc.subject.local | Surface plasmon resonance imaging | - |
dc.subject.local | surface plasmon resonance (SPR) imaging | - |
dc.description.journalClass | Y | - |
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