Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes

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Title
Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes
Author(s)
Deog Bon Koo; Jung IL Chae; Ji Su Kim; Gabbine Wee; Bong Seok Song; Kyung Kwang Lee; Yong Mahn Han
Bibliographic Citation
Molecular Reproduction and Development, vol. 72, no. 4, pp. 542-549
Publication Year
2005
Abstract
This study was conducted to examine the activities of maturation-promoting factor (MPF) and mitogen-activated protein (MAP) kinase in the porcine oocytes after artificial activation. To determine optimal electrical activation condition, oocytes were exposed to single DC pulse in a variety of electric field strengths (120, 150, 180, and 210 V/mm) and pulse durations (15, 30, 45, and 60 μsec). After the artificial activation, 40-50 oocytes were cultured in a 50 μl drop of NCSU23 medium supplemented with 0.4% BSA at 39°C, 5% CO2 in air for 6 days. No difference was detected in the preimplantation development of pocine oocytes and the mean nuclei number of blastocysts between electric field strengths. Under the 180 V/mm electric field strength, short pulse durations (15 and 30 μsec) showed a higher preimplantation developmental rate of the oocytes and mean nuclei number of blastocysts than an extended electric pulse (60 μsec) (P < 0.05). Single electrical stimulus (180 V/mm, 15 μusec) resulted in higher preimplantation development of porcine oocytes as compared to other chemical stimulators (P < 0.01). Western blot analyses showed the decrease of MPF and MAP kinase in the electrically-activated oocytes. After single electrical stimulus, the amounts of both cdc2 and ERK in porcine oocytes were remarkably reduced by 4 hr and then further decreased by 8 hr. However, the chemically-stimulated oocytes did not show any significant change at the levels of MPF and MAP kinase. Our results indicate that the optimal single electrical pulse is effective on the inactivation of MPF and MAP kinase, eventually leading to the parthenogenetic development of porcine oocytes.
Keyword
ActivationMAP kinaseMPFPorcine oocytesSingle electrical stimulus
ISSN
1040-452X
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/mrd.20382
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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